PMID- 15671337
OWN - NLM
STAT- MEDLINE
DCOM- 20050330
LR  - 20131121
IS  - 0022-0949 (Print)
IS  - 0022-0949 (Linking)
VI  - 208
IP  - Pt 3
DP  - 2005 Feb
TI  - A distinct carbonic anhydrase in the mucus of the colon of humans and other 
      mammals.
PG  - 487-96
AB  - We have collected gastrointestinal, mainly colonic, mucus from humans, guinea 
      pigs, rats, and normal and carbonic anhydrase II (CAII)-deficient mice. In the 
      mucus of all species, substantial CA activity was present. Using antibodies 
      against human CA isoforms we found that the human mucus CA differs from cytosolic 
      CAI and CAII, membrane-bound CAIV, and the secreted CAVI of saliva. The high 
      sensitivity of mucus CA to acetazolamide rules out its identity with cytosolic 
      CAIII. Partial sequences obtained from the purified human mucus CA show 
      similarity, but not identity, with human CAI, and clear differences from the 
      other known CAs. Additional evidence concerning the CA isoform present in mucus 
      was obtained for the mucus CA of other species and was derived from: (1) the 
      mucus of CAII-deficient mice, whose high CA activity confirms that it is not CAII 
      that is responsible; (2) the inhibitory effect of iodide, which shows that mucus 
      CA from mice, guinea pig and humans does not have the high anion sensitivity of 
      CAI; (3) the inactivating effect of 0.2% SDS on guinea pig, mouse and human mucus 
      CA, ruling out the SDS-resistant CAIV; and (4) the partitioning of guinea-pig 
      mucus CA into the water phase in Triton X114 phase separation experiments, which 
      also argues against its identity with membrane-bound CAs, such as CAIV. A 
      comparison of colonic mucus CA activity in normal and germ-free rats indicates 
      that the mucus CA is not of bacterial origin but is produced by the 
      gastrointestinal tissues. We conclude (from its immunoreactivity, from iodide 
      inhibition and from partial amino acid sequences) that mucus CA of human origin 
      probably represents an isozyme, which is specific for mucus and is not identical 
      with the known CA isozymes. The results obtained for mucus CA of other species 
      collectively point in the same direction.
FAU - Kleinke, Tanja
AU  - Kleinke T
AD  - Zentrum Physiologie, Medizinische Hochschule Hannover, D-30625 Hannover, Germany.
FAU - Wagner, Siegfried
AU  - Wagner S
FAU - John, Harald
AU  - John H
FAU - Hewett-Emmett, David
AU  - Hewett-Emmett D
FAU - Parkkila, Seppo
AU  - Parkkila S
FAU - Forssmann, Wolf-Georg
AU  - Forssmann WG
FAU - Gros, Gerolf
AU  - Gros G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Biol
JT  - The Journal of experimental biology
JID - 0243705
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - O3FX965V0I (Acetazolamide)
SB  - IM
MH  - Acetazolamide/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carbonic Anhydrases/chemistry/*metabolism
MH  - Colon/enzymology
MH  - Female
MH  - Gastrointestinal Tract/*enzymology
MH  - Germ-Free Life
MH  - Guinea Pigs
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Mucus/*enzymology
MH  - Phylogeny
MH  - Rats
MH  - Rats, Wistar
MH  - Sequence Homology, Amino Acid
EDAT- 2005/01/27 09:00
MHDA- 2005/03/31 09:00
CRDT- 2005/01/27 09:00
PHST- 2005/01/27 09:00 [pubmed]
PHST- 2005/03/31 09:00 [medline]
PHST- 2005/01/27 09:00 [entrez]
AID - 208/3/487 [pii]
AID - 10.1242/jeb.01398 [doi]
PST - ppublish
SO  - J Exp Biol. 2005 Feb;208(Pt 3):487-96. doi: 10.1242/jeb.01398.