PMID- 15671757
OWN - NLM
STAT- MEDLINE
DCOM- 20050225
LR  - 20171116
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 17
IP  - 4
DP  - 2004
TI  - Temporary CD8+ T-cell depletion in pigs does not exacerbate infection with 
      porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 594-603
AB  - Several studies have demonstrated a consistent increase in the CD8+ T-cell subset 
      of pigs following infection with porcine reproductive and respiratory virus 
      (PRRSV). Consequently, it has been suggested that CD8+ T-cells may play an 
      important role in protection against this infection. In order to test this 
      hypothesis, we examined five 5-week-old pigs, which had been depleted for CD8+ 
      T-cells by treatment with anti-CD8 mAb injections, starting 2 days before 
      inoculation with PRRSV. Virus-inoculated and sham-inoculated age-matched pigs 
      served as controls. Blood samples were collected continuously, together with 
      organ material at necropsy, to study kinetics of leukocyte subpopulations, 
      antibody production and virus persistence in individual pigs. Significant lower 
      CD8+ T-cell counts on day 0, that is, before virus challenge, in the anti-CD8 mAb 
      treated pigs compared to the control pigs, confirmed the depletion effect of 
      specific mAb therapy. Almost complete depletion of cell subsets expressing the 
      CD8+ antigen was obtained on day 2 and 5 post infection (PI) with nadir less than 
      1% of peripheral blood mononuclear cells (PBMC). One week PI, an increase in 
      T-cell subsets was observed for both anti-CD8 mAb treated pigs and 
      virus-inoculated control pigs. T-memory cells and cytotoxic T-cells reached 
      levels comparative with the virus-inoculated control pigs on days 8 and 12 PI, 
      respectively, whereas NKcells remained suppressed for the rest of the 
      experimental period. An extraordinary increase of T-helper cells in the anti-CD8 
      mAb treated pigs with a significantly higher level than in the virus-inoculated 
      and sham-inoculated control pigs, was observed from day 12 PI. In conclusion, it 
      was established that CD8+ T-cell depletion in the early phase of PRRSV infection 
      neither caused increased disease nor influenced the ability to clear virus in the 
      treated pigs.
FAU - Lohse, Louise
AU  - Lohse L
AD  - Department of Virology, Danish Institute for Food and Veterinary Research, 
      Lindholm, DK-4771 Kalvehave, Denmark.
FAU - Nielsen, Jens
AU  - Nielsen J
FAU - Eriksen, Lis
AU  - Eriksen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (CD8 Antigens)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/administration & dosage/immunology
MH  - Antibodies, Viral/blood
MH  - CD8 Antigens/immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Lymphocyte Depletion/*methods
MH  - Porcine Reproductive and Respiratory 
      Syndrome/*immunology/*physiopathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*pathogenicity
MH  - Swine
MH  - Swine Diseases/immunology/virology
MH  - Viremia/immunology/veterinary/virology
EDAT- 2005/01/27 09:00
MHDA- 2005/02/26 09:00
CRDT- 2005/01/27 09:00
PHST- 2005/01/27 09:00 [pubmed]
PHST- 2005/02/26 09:00 [medline]
PHST- 2005/01/27 09:00 [entrez]
AID - 10.1089/vim.2004.17.594 [doi]
PST - ppublish
SO  - Viral Immunol. 2004;17(4):594-603. doi: 10.1089/vim.2004.17.594.