PMID- 15672476
OWN - NLM
STAT- MEDLINE
DCOM- 20050303
LR  - 20171116
IS  - 1613-4125 (Print)
IS  - 1613-4125 (Linking)
VI  - 48
IP  - 5
DP  - 2004 Oct
TI  - Cow's milk allergens identification by two-dimensional immunoblotting and mass 
      spectrometry.
PG  - 363-9
AB  - Cow's milk allergy (CMA) has become a common disease in early childhood, its 
      prevalence ranging from 1.6% to 2.8% among children younger than 2 years of age. 
      The role of different cow's milk protein (CMP) in the pathogenesis of CMA is 
      still controversial. Even if the proteins most frequently and most intensively 
      recognized by immunoglobulin E (IgE) seem to be the most abundant in milk 
      (caseins and beta-lactoglobulin), with an although great variability all milk 
      proteins appear to be potential allergens, even those that are present in trace 
      amounts (i.e., lactoferrin, IgG, and BSA). In this work proteomics techniques 
      have been applied for CMP allergens analysis. Allergens have been identified by 
      immunoblotting following resolution of CMP components by two-dimensional 
      electrophoresis. Sera from 20 milk-allergic subjects, as proven by oral 
      provocation test, CAP-RAST and skin prick test, have been used for cow's milk 
      major allergen identification. Cow's milk proteins and their isoforms were 
      identified by matrix assisted laser desorption/ionization-time of flight 
      (MALDI-TOF)-mass spectrometry. In our group of patients, the prevalence of CMP 
      allergens, i.e., the total number of subjects sensitized to CMP divided by the 
      total number of the subjects enrolled in the study, was: 55% alpha(s1)-casein, 
      90% alpha(s2)-casein, 15% beta-casein, 50% kappa-casein, 45% beta-lactoglobulin, 
      45% BSA, 95% IgG-heavy chain, 50% lactoferrin, and 0% alpha-lactalbumin.
FAU - Natale, Massimo
AU  - Natale M
AD  - CNR-lnstitute of Science of Food Production, Section of Torino, Italy.
FAU - Bisson, Carolina
AU  - Bisson C
FAU - Monti, Giovanna
AU  - Monti G
FAU - Peltran, Amalia
AU  - Peltran A
FAU - Garoffo, Lorenza Perono
AU  - Garoffo LP
FAU - Valentini, Sabina
AU  - Valentini S
FAU - Fabris, Claudio
AU  - Fabris C
FAU - Bertino, Enrico
AU  - Bertino E
FAU - Coscia, Alessandra
AU  - Coscia A
FAU - Conti, Amedeo
AU  - Conti A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Allergens)
RN  - 0 (Caseins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Lactoglobulins)
RN  - 0 (Milk Proteins)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9013-90-5 (Lactalbumin)
RN  - EC 3.4.21.- (Lactoferrin)
SB  - IM
MH  - Allergens/*analysis/immunology
MH  - Animals
MH  - Caseins/immunology
MH  - Cattle
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/immunology
MH  - Immunoglobulin G/immunology
MH  - Immunoglobulin Heavy Chains/immunology
MH  - Infant
MH  - Lactalbumin/immunology
MH  - Lactoferrin/immunology
MH  - Lactoglobulins/immunology
MH  - Male
MH  - Milk/*immunology
MH  - Milk Hypersensitivity/diagnosis/*immunology
MH  - Milk Proteins/*immunology
MH  - Serum Albumin, Bovine/immunology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2005/01/27 09:00
MHDA- 2005/03/04 09:00
CRDT- 2005/01/27 09:00
PHST- 2005/01/27 09:00 [pubmed]
PHST- 2005/03/04 09:00 [medline]
PHST- 2005/01/27 09:00 [entrez]
AID - 10.1002/mnfr.200400011 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2004 Oct;48(5):363-9. doi: 10.1002/mnfr.200400011.