PMID- 15674338
OWN - NLM
STAT- MEDLINE
DCOM- 20050324
LR  - 20081121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 24
IP  - 10
DP  - 2005 Mar 3
TI  - Retinoblastoma susceptibility gene product pRB activates hypoxia-inducible 
      factor-1 (HIF-1).
PG  - 1802-8
AB  - Hypoxia-inducible factor-1 alpha (HIF-1alpha) constitutes a regulatory subunit of 
      HIF-1, a major transcriptional activator of genes that coordinate physiological 
      and pathological responses towards hypoxia. In order to identify novel 
      interaction partners of HIF-1alpha we have applied T7 phage display system and 
      identified a domain inherent in the retinoblastoma protein (pRB). The interaction 
      between pRB and HIF-1alpha was confirmed by in vitro experiments and in 
      transfected cells. Thereby, an HIF-1alpha domain spanning amino acids 530-694 was 
      mapped to be required for pRB binding. Overexpression of pRB provoked 
      transcriptional activation of HIF-1alpha under normoxia. Furthermore, the domain 
      of pRB identified to bind HIF-1alpha in vitro is sufficient to cause HIF-1alpha 
      transcriptional activation with the further notion that phosphorylation deficient 
      pRB shows stronger HIF-1alpha transactivation. Using ChIP analysis, we show that 
      HIF-1alpha responsive elements (HREs) are precipitated using alpha-pRB 
      antibodies. Additionally, a functional interaction between pRB and HIF-1alpha is 
      confirmed by showing that HIF-1alpha reverses the transcription repressor 
      function of pRB.
FAU - Budde, Andreja
AU  - Budde A
AD  - Department of Medicine IV-Experimental Division, Faculty of Medicine, University 
      of Erlangen-Nurnberg, 91054 Erlangen, Germany.
FAU - Schneiderhan-Marra, Nicole
AU  - Schneiderhan-Marra N
FAU - Petersen, Gabriele
AU  - Petersen G
FAU - Brune, Bernhard
AU  - Brune B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E2F Transcription Factors)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Cycle Proteins/physiology
MH  - DNA-Binding Proteins/physiology
MH  - E2F Transcription Factors
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Molecular Sequence Data
MH  - Response Elements/physiology
MH  - Retinoblastoma Protein/chemistry/*physiology
MH  - Transcription Factors/chemistry/*physiology
MH  - Transcriptional Activation
EDAT- 2005/01/28 09:00
MHDA- 2005/03/25 09:00
CRDT- 2005/01/28 09:00
PHST- 2005/01/28 09:00 [pubmed]
PHST- 2005/03/25 09:00 [medline]
PHST- 2005/01/28 09:00 [entrez]
AID - 1208369 [pii]
AID - 10.1038/sj.onc.1208369 [doi]
PST - ppublish
SO  - Oncogene. 2005 Mar 3;24(10):1802-8. doi: 10.1038/sj.onc.1208369.