PMID- 15674345
OWN - NLM
STAT- MEDLINE
DCOM- 20050322
LR  - 20071114
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 24
IP  - 9
DP  - 2005 Feb 24
TI  - Centromeric chromosomal translocations show tissue-specific differences between 
      squamous cell carcinomas and adenocarcinomas.
PG  - 1571-9
AB  - Structural chromosomal aberrations are common in epithelial tumors. Here, we 
      compared the location of centromeric breaks associated with whole arm 
      translocations in seven adenocarcinoma cell lines and nine squamous cell 
      carcinoma cell lines using SKY, microarray-based comparative genomic 
      hybridization (array CGH) and fluorescence in situ hybridization (FISH). Whole 
      arm translocations were more frequent in squamous cell carcinomas (112 in nine 
      cell lines and nine in one short-term culture) than in adenocarcinomas (13 in 
      seven cases) and most often resulted in copy number alterations. Array CGH 
      analysis demonstrated that in all squamous cell carcinomas and in most 
      adenocarcinomas, the breakpoints of unbalanced whole arm translocations occurred 
      between the two clones on the array flanking the centromeres. However, FISH with 
      centromeric probes revealed that in squamous cell carcinomas, the marker 
      chromosomes with whole arm translocations contained centromeres comprised of 
      material from both participating chromosomes, while in adenocarcinomas 
      centromeric material from only one of the chromosomes was present. These 
      observations suggest that different mechanisms of centromeric instability 
      underlie the formation of chromosomal aberrations in adenocarcinomas and squamous 
      cell carcinomas.
FAU - Hermsen, Mario
AU  - Hermsen M
AD  - Department of Pathology, VU University Medical Center, Amsterdam, The 
      Netherlands. mhermsen@hca.es
FAU - Snijders, Antoine
AU  - Snijders A
FAU - Guervos, Marta Alonso
AU  - Guervos MA
FAU - Taenzer, Simone
AU  - Taenzer S
FAU - Koerner, Ulrike
AU  - Koerner U
FAU - Baak, Jan
AU  - Baak J
FAU - Pinkel, Daniel
AU  - Pinkel D
FAU - Albertson, Donna
AU  - Albertson D
FAU - van Diest, Paul
AU  - van Diest P
FAU - Meijer, Gerrit
AU  - Meijer G
FAU - Schrock, Evelin
AU  - Schrock E
LA  - eng
GR  - CA94407/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Centromere/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human/*genetics
MH  - Diagnosis, Differential
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Array Sequence Analysis
MH  - *Translocation, Genetic
EDAT- 2005/01/28 09:00
MHDA- 2005/03/23 09:00
CRDT- 2005/01/28 09:00
PHST- 2005/01/28 09:00 [pubmed]
PHST- 2005/03/23 09:00 [medline]
PHST- 2005/01/28 09:00 [entrez]
AID - 1208294 [pii]
AID - 10.1038/sj.onc.1208294 [doi]
PST - ppublish
SO  - Oncogene. 2005 Feb 24;24(9):1571-9. doi: 10.1038/sj.onc.1208294.