PMID- 15676219
OWN - NLM
STAT- MEDLINE
DCOM- 20050411
LR  - 20071114
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 33
IP  - 2
DP  - 2005 Feb
TI  - Genetic regulation of hematopoietic stem cell exhaustion during development and 
      growth.
PG  - 243-50
AB  - OBJECTIVE: During aging, hematopoietic stem cell (HSC) exhaustion is more severe 
      in BALB/cByJ (BALB) mice than in C57BL/6J (B6) mice. Our objective is to 
      determine whether HSC exhaustion during development from fetus to adult also is 
      more severe for BALB than for B6 mice. MATERIALS AND METHODS: Hematopoietic stem 
      cells from fetal liver cells (FLCs) and from young adult bone marrow cells (BMCs) 
      were compared using the competitive repopulation assay to measure long-term 
      repopulating ability (LTRA) and HSC expansion after serial transplantation. LTRAs 
      were measured in repopulating units (RU), as the ability to produce donor-type 
      erythrocytes and lymphocytes in lethally irradiated recipients relative to the 
      congenic fresh marrow competitor. To test expansion, FLCs or BMCs were serially 
      transplanted into lethally irradiated carriers whose marrow cells were compared 
      using fluorescence-activated cell staining (FACS), and subsequently tested for 
      LTRA. RESULTS: BALB and B6 FLCs, respectively, repopulated 2.6 and 13.5 times as 
      well as BMCs. LTRAs correlated with HSC expansion for BALB, but not B6. Per 
      million donor cells, CD34(-) HSC-enriched fractions (HEFs) and total RU values 
      were 6.8 and 4.6 times higher for FLCs than for BMCs in BALB carriers, while 
      these ratios were only 1.2 and 0.97 higher in B6 carriers. CONCLUSION: In B6 HSC 
      development, LTRA is dissociated from expansion. Although 1 x 10(6) BMCs have 
      much lower LTRA, they expand HSCs as well as 1 x 10(6) FLCs. HSC expansion is 
      partly exhausted in BALB, but not B6, during development.
FAU - Yuan, Rong
AU  - Yuan R
AD  - The Jackson Laboratory, Bar Harbor, ME, USA.
FAU - Astle, C M
AU  - Astle CM
FAU - Chen, Jichun
AU  - Chen J
FAU - Harrison, David E
AU  - Harrison DE
LA  - eng
GR  - AG18003/AG/NIA NIH HHS/United States
GR  - CA34196/CA/NCI NIH HHS/United States
GR  - HL58820/HL/NHLBI NIH HHS/United States
GR  - HL62320/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - *Gene Expression Regulation
MH  - Hematopoietic Stem Cells/*cytology
MH  - Liver/cytology/embryology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Species Specificity
EDAT- 2005/01/29 09:00
MHDA- 2005/04/12 09:00
CRDT- 2005/01/29 09:00
PHST- 2004/09/14 00:00 [received]
PHST- 2004/10/21 00:00 [accepted]
PHST- 2005/01/29 09:00 [pubmed]
PHST- 2005/04/12 09:00 [medline]
PHST- 2005/01/29 09:00 [entrez]
AID - S0301-472X(04)00388-1 [pii]
AID - 10.1016/j.exphem.2004.10.014 [doi]
PST - ppublish
SO  - Exp Hematol. 2005 Feb;33(2):243-50. doi: 10.1016/j.exphem.2004.10.014.