PMID- 15677796
OWN - NLM
STAT- MEDLINE
DCOM- 20050531
LR  - 20190516
IS  - 0149-5992 (Print)
IS  - 0149-5992 (Linking)
VI  - 28
IP  - 2
DP  - 2005 Feb
TI  - Chronic vascular inflammation in patients with type 2 diabetes: endothelial 
      biopsy and RT-PCR analysis.
PG  - 379-84
AB  - OBJECTIVE: Chronic vascular inflammation may play a role in the development of 
      macrovascular complications in diabetic patients. In this study, we examine the 
      association of endothelial expression of two inflammatory mediators, receptor for 
      advanced glycation end product (RAGE) and monocyte chemoattractant protein-1 
      (MCP-1), with type 2 diabetes using novel endothelial biopsy and RT-PCR 
      techniques. RESEARCH DESIGN AND METHODS: Endothelial samples are obtained from 
      the aorta of 12 patients with type 2 diabetes and 23 control subjects who 
      underwent cardiac catheterization for chest pain syndrome or heart transplant 
      follow-up. Endothelial cells are purified using magnetic beads with adsorbed 
      CD146 antibody and subjected to RT-PCR analysis of RAGE and MCP-1 transcripts. 
      The association of RAGE and MCP-1 expression with type 2 diabetes is assessed 
      with chi(2) test and confirmed with in vitro experiments on human aorta 
      endothelial cells. RESULTS: RT-PCR reveals gene expression patterns in 
      patient-derived endothelial cells. Strong associations are observed between 
      induction of RAGE mRNA and diabetes (P < 0.01) and between induction of RAGE and 
      MCP-1 transcripts (P < 0.05). Treatment of cultured human aortic endothelial 
      cells with S100b induces the expression of MCP-1 and RAGE transcripts. 
      CONCLUSIONS: Endothelial cells can be harvested during cardiac catheterization 
      and can be characterized with respect to molecular phenotypes under the influence 
      of both genetic and environmental factors. Induction of RAGE and MCP-1 
      transcripts in patients with diabetes supports a role of chronic vascular 
      inflammation in macrovascular complications.
FAU - Feng, Lei
AU  - Feng L
AD  - Department of Radiology, New York Presbyterian Hospital, 177 Fort Washington 
      Ave., MHB 8SK, New York, NY 10032, USA. lf66@columbia.edu
FAU - Matsumoto, Carolyn
AU  - Matsumoto C
FAU - Schwartz, Allan
AU  - Schwartz A
FAU - Schmidt, Ann Marie
AU  - Schmidt AM
FAU - Stern, David M
AU  - Stern DM
FAU - Pile-Spellman, John
AU  - Pile-Spellman J
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diabetes Care
JT  - Diabetes care
JID - 7805975
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Aged
MH  - Aorta/cytology
MH  - Biopsy/*methods
MH  - Cardiac Catheterization
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics
MH  - Chronic Disease
MH  - Diabetes Mellitus, Type 2/genetics/*pathology
MH  - Diabetic Angiopathies/genetics/*pathology
MH  - Endothelium, Vascular/*pathology/physiology
MH  - Humans
MH  - Middle Aged
MH  - Pilot Projects
MH  - Receptor for Advanced Glycation End Products
MH  - Receptors, Immunologic/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vasculitis/genetics/*pathology
EDAT- 2005/01/29 09:00
MHDA- 2005/06/01 09:00
CRDT- 2005/01/29 09:00
PHST- 2005/01/29 09:00 [pubmed]
PHST- 2005/06/01 09:00 [medline]
PHST- 2005/01/29 09:00 [entrez]
AID - 28/2/379 [pii]
AID - 10.2337/diacare.28.2.379 [doi]
PST - ppublish
SO  - Diabetes Care. 2005 Feb;28(2):379-84. doi: 10.2337/diacare.28.2.379.