PMID- 15677904
OWN - NLM
STAT- MEDLINE
DCOM- 20050512
LR  - 20190513
IS  - 1078-0998 (Print)
IS  - 1078-0998 (Linking)
VI  - 11
IP  - 2
DP  - 2005 Feb
TI  - Evaluation of surgical tissue from patients with Crohn's disease for the presence 
      of Mycobacterium avium subspecies paratuberculosis DNA by in situ hybridization 
      and nested polymerase chain reaction.
PG  - 116-25
AB  - Crohn's disease (CD) is a chronic inflammatory bowel disease (IBD) with tissue 
      granuloma and histopathological alteration that resembles aspects in 
      tuberculosis, leprosy, and paratuberculosis. Mycobacterium avium subsp 
      paratuberculosis (MAP) is the causative agent of paratuberculosis, with a 
      suspected role in the etiology of CD. We investigated the presence of MAP DNA in 
      31 surgical tissue samples from 20 subjects using fluorescence in situ 
      hybridization (FISH) with the aid of confocal scanning laser microscopy and 
      nested polymerase chain reaction (PCR) using the IS900 sequence unique to MAP. 
      MAP DNA was detected by PCR in tissue from 10 of 12 (83%) patients with CD: 7/12 
      (58%) in inflamed, 6/11 (55%) in noninflamed and in 10 (83%) of either tissue and 
      by FISH in 8 of 12 (67%) patients with CD: 7 of 12 (58%) in inflamed, 4 of 11 
      (36%) in noninflamed, and in 8(67%) of either tissue. In non-IBD subjects, MAP 
      DNA was detected in the tissue of only 1 of 6 patients (17%) by PCR and 0 of 6 
      patients (0%) by FISH. MAP DNA was identified by PCR in inflamed tissue from 2 of 
      2 patients with ulcerative colitis. The detection of MAP DNA by either technique 
      in tissue from subjects with CD is significant compared with non-IBD subjects (P 
      < 0.005). Identification of MAP DNA in both inflamed and noninflamed tissue by 
      both techniques suggests that MAP infection in patients with CD may be systemic. 
      The data add more evidence toward a possible association of MAP in the 
      pathogenesis of CD.
FAU - Romero, Claudia
AU  - Romero C
AD  - Department of Molecular Biology and Microbiology and Biomolecular Science Center, 
      The Burnett College of Biomedical Sciences, University of Central Florida, 
      Orlando, Florida 32816, USA.
FAU - Hamdi, Amal
AU  - Hamdi A
FAU - Valentine, John F
AU  - Valentine JF
FAU - Naser, Saleh A
AU  - Naser SA
LA  - eng
GR  - R01-AI51251-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Inflamm Bowel Dis
JT  - Inflammatory bowel diseases
JID - 9508162
RN  - 0 (DNA, Bacterial)
SB  - IM
CIN - Inflamm Bowel Dis. 2005 Aug;11(8):782-3. PMID: 16043998
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Crohn Disease/*microbiology/pathology/surgery
MH  - DNA, Bacterial/*analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium avium subsp. paratuberculosis/*genetics/isolation & 
      purification/pathogenicity
MH  - Polymerase Chain Reaction
EDAT- 2005/01/29 09:00
MHDA- 2005/05/13 09:00
CRDT- 2005/01/29 09:00
PHST- 2005/01/29 09:00 [pubmed]
PHST- 2005/05/13 09:00 [medline]
PHST- 2005/01/29 09:00 [entrez]
AID - 00054725-200502000-00004 [pii]
AID - 10.1097/00054725-200502000-00004 [doi]
PST - ppublish
SO  - Inflamm Bowel Dis. 2005 Feb;11(2):116-25. doi: 10.1097/00054725-200502000-00004.