PMID- 15678807
OWN - NLM
STAT- MEDLINE
DCOM- 20050215
LR  - 20061115
IS  - 0120-4157 (Print)
IS  - 0120-4157 (Linking)
VI  - 24
IP  - 4
DP  - 2004 Dec
TI  - [Genetic manipulation and the study of the protozoan parasite Leishmania].
PG  - 438-55
AB  - During the last 15 years, many aspects of the functional genomics of Leishmania 
      have been revealed due to advances in DNA transfection, gene disruption and 
      complementation through homologous recombination, and efficient strategies for 
      the selection of transfected cells. These strategies have provided information 
      about gene expression and protein function in the context of the intact parasite. 
      The genome of Leishmania shows a marked deficiency of known transcription 
      initiation factors, and gene expression is regulated almost entirely at the 
      post-transcriptional level through trans-splicing of mRNAs and novel control 
      mechanisms involving differential processing of 3'-untranslated regions (3'-UTRs) 
      of mRNAs. Therefore, gene transfection represents a useful tool for the 
      identification and functional analysis of genes of interest as well as the 
      mechanisms that direct their regulation. The development of genetic manipulation 
      systems has provided opportunities for the study of genes involved in virulence, 
      intracellular survival and drug resistance of Leishmania, as well as for the 
      functional validation of specific parasite proteins as new chemo- and 
      immunotherapeutic targets. The current review presents recent advances in genetic 
      manipulations that permit structural, functional and phenotypic analyses and by 
      means of gene deletion and complementation using the methods of gene 
      transfection.
FAU - Cortazar, Tania M
AU  - Cortazar TM
AD  - Centro Internacional de Entrenamiento e Investigaciones Medicas, CIDEIM, Cali, 
      Colombia.
FAU - Walker, John
AU  - Walker J
LA  - spa
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
TT  - Manipulacion genetica y el estudio del parasito protozoario Leishmania.
PL  - Colombia
TA  - Biomedica
JT  - Biomedica : revista del Instituto Nacional de Salud
JID - 8205605
RN  - 0 (DNA, Protozoan)
SB  - IM
MH  - Animals
MH  - *DNA, Protozoan
MH  - *Gene Expression Regulation
MH  - Leishmania/*genetics
MH  - *Transfection
RF  - 121
EDAT- 2005/02/01 09:00
MHDA- 2005/02/16 09:00
CRDT- 2005/02/01 09:00
PHST- 2005/02/01 09:00 [pubmed]
PHST- 2005/02/16 09:00 [medline]
PHST- 2005/02/01 09:00 [entrez]
PST - ppublish
SO  - Biomedica. 2004 Dec;24(4):438-55.