PMID- 15679584
OWN - NLM
STAT- MEDLINE
DCOM- 20050613
LR  - 20061115
IS  - 0906-6705 (Print)
IS  - 0906-6705 (Linking)
VI  - 14
IP  - 2
DP  - 2005 Feb
TI  - UVB-induced leucocyte trafficking in the epidermis of photosensitive lupus 
      erythematosus patients: normal depletion of Langerhans cells.
PG  - 138-42
AB  - BACKGROUND: The pathogenic mechanisms of UV-induced skin lesions of lupus 
      erythematosus (LE) are unknown. In a recent study of pathogenic mechanisms of 
      polymorphic light eruption (PLE), significantly more Langerhans cells (LCs) 
      persisted in the epidermis after UVB overexposure than in healthy individuals. 
      Interestingly, the same phenomenon was observed in one subacute cutaneous lupus 
      erythematosus (SCLE) patient. It could therefore be hypothesized that both 
      photodermatoses share a common pathogenic mechanism of photosensitivity. In the 
      present study, we tested this hypothesis by investigating leucocyte trafficking 
      in the initial phase of cutaneous LE after intense UVB exposure. METHODS: In 22 
      photosensitive LE patients (12 chronic discoid lupus erythematosus, seven 
      systemic lupus erythematosus and three SCLE) and nine age/sex-matched controls, 
      uninvolved buttock skin was exposed to six minimal erythemal dose (MED) UVB 
      radiation. Subsequently, biopsies were taken after 24, 48 and 72 h, and one 
      control biopsy was taken from unirradiated skin. Skin sections were stained for 
      the presence of LCs, neutrophils and macrophages. Areal percentages of positively 
      stained cells within the epidermis were quantified and compared between the 
      patients and controls. RESULTS: A gradual decrease of epidermal LCs and a gradual 
      increase of epidermal neutrophils and macrophages at several timepoints after six 
      MED irradiation was observed equally in both LE patients and controls. 
      CONCLUSION: Immunohistopathology of irradiated uninvolved skin of photosensitive 
      LE patients did not reveal the same pathologic trafficking of LCs and neutrophils 
      as described for PLE patients. We conclude that different mechanisms are 
      operative in the pathogenesis of PLE and photosensitive LE.
FAU - Janssens, Artiena Soe
AU  - Janssens AS
AD  - Department of Dermatology, Leiden University Medical Center, Leiden, The 
      Netherlands. a.s.janssens@lumc.nl
FAU - Lashley, Eileen E L O
AU  - Lashley EE
FAU - Out-Luiting, Coby J
AU  - Out-Luiting CJ
FAU - Willemze, Rein
AU  - Willemze R
FAU - Pavel, Stan
AU  - Pavel S
FAU - de Gruijl, Frank R
AU  - de Gruijl FR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Epidermis/pathology/*radiation effects
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Langerhans Cells/pathology/*radiation effects
MH  - Leukocytes/cytology/*radiation effects
MH  - Light
MH  - Lupus Erythematosus, Cutaneous/*metabolism/*pathology
MH  - Lupus Erythematosus, Systemic/*metabolism/*pathology
MH  - Male
MH  - Middle Aged
MH  - Photosensitivity Disorders/*pathology
MH  - Skin/pathology
MH  - Time Factors
MH  - Ultraviolet Rays
EDAT- 2005/02/01 09:00
MHDA- 2005/06/14 09:00
CRDT- 2005/02/01 09:00
PHST- 2005/02/01 09:00 [pubmed]
PHST- 2005/06/14 09:00 [medline]
PHST- 2005/02/01 09:00 [entrez]
AID - EXD279 [pii]
AID - 10.1111/j.0906-6705.2005.00279.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2005 Feb;14(2):138-42. doi: 10.1111/j.0906-6705.2005.00279.x.