PMID- 15680701
OWN - NLM
STAT- MEDLINE
DCOM- 20050425
LR  - 20061115
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 131
IP  - 1
DP  - 2005
TI  - Proliferation, migration, and differentiation of endogenous ependymal region 
      stem/progenitor cells following minimal spinal cord injury in the adult rat.
PG  - 177-87
AB  - Ependymal cells of the adult mammalian spinal cord exhibit stem/progenitor cell 
      properties following injury. In the present study, we utilized intraventricular 
      injection of 
      1,1'-dioctadecyl-6,6'-di(4-sulfophenyl)-3,3,3',3'-tetramethylindocarbocyanine 
      (DiI) to label the ependyma lining the central canal to allow tracking of the 
      migration of endogenous ependymal cells and their progeny after spinal cord 
      injury (SCI). We developed a minimal injury model that preserved the integrity of 
      the central canal and did not interfere with ependymal cell labeling. Three days 
      following SCI, there was an 8.6-fold increase in the proliferative labeling index 
      of the ependymal cells at the level of the needle track based on 
      bromodeoxyuridine labeling, compared with 1 day post-injury. Terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) positive 
      cells were not detected in the ependyma or surrounding gray matter, indicating 
      that ependymal cells do not undergo apoptosis in response to minimal injury. 
      Nestin was rapidly induced in the ependyma by 1 day and expression peaked by 7 
      days post-injury. We quantitated the number and distance of ependymal cell 
      migration following minimal injury. The number of ependymal cells migrating from 
      the region of the central canal increased by 3 days following minimal injury and 
      DiI-labeled glial fibrillary acidic protein expressing cells were detected 14 
      days post-SCI, most of which migrated within 70 microm of the region of the 
      central canal. These results show that a minimal SCI adjacent to the ependyma is 
      sufficient to induce an endogenous ependymal cell response where ependymal 
      stem/progenitor cells proliferate and migrate from the region of the central 
      canal, differentiating primarily into astrocytes.
FAU - Mothe, A J
AU  - Mothe AJ
AD  - Toronto Western Research Institute and Krembil Neuroscience Centre, McL 12-423, 
      399 Bathurst Street, Toronto, Ontario, Canada M5T 2S8.
FAU - Tator, C H
AU  - Tator CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Differentiation
MH  - Cell Division
MH  - Cell Movement
MH  - Cerebral Ventricles/physiology/physiopathology
MH  - Disease Models, Animal
MH  - Ependyma/pathology/physiology/*physiopathology
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Rats
MH  - Spinal Cord Injuries/pathology/*physiopathology
MH  - Stem Cells/cytology/pathology/*physiology
EDAT- 2005/02/01 09:00
MHDA- 2005/04/26 09:00
CRDT- 2005/02/01 09:00
PHST- 2004/10/06 00:00 [accepted]
PHST- 2005/02/01 09:00 [pubmed]
PHST- 2005/04/26 09:00 [medline]
PHST- 2005/02/01 09:00 [entrez]
AID - S0306-4522(04)00937-6 [pii]
AID - 10.1016/j.neuroscience.2004.10.011 [doi]
PST - ppublish
SO  - Neuroscience. 2005;131(1):177-87. doi: 10.1016/j.neuroscience.2004.10.011.