PMID- 15681745
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20230120
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 1029
DP  - 2004 Dec
TI  - Intestinal epithelial cells control dendritic cell function.
PG  - 66-74
AB  - Dendritic cells (DCs) comprise a family of cells specializing in antigen capture 
      and presentation to T cells. We have recently shown that DC play an active role 
      in bacterial uptake across mucosal surfaces. Indeed, DC are able to open tight 
      junctions and to sample antigens directly across epithelia, both in vitro and in 
      vivo. Because DC express tight junction proteins, the integrity of the epithelial 
      barrier is preserved. In this study we have analyzed the possible involvement of 
      epithelial cells in controlling DC function. We developed an in vitro model in 
      our laboratory consisting of a three-player system of dendritic cells, epithelial 
      cell monolayers, and bacteria. The crosstalk between epithelial cells and 
      dendritic cells was analyzed, and epithelial cells were tested for their capacity 
      to release cytokines and chemokines that induce the migration and activation of 
      DC. We show that the capacity of epithelial cells to produce cytokines and 
      activate DC is dependent on the invasiveness of the bacteria tested. In 
      particular, invasive bacteria stimulate epithelial cells to release 
      proinflammatory cytokines and to induce the maturation state of DC. By contrast, 
      noninvasive bacteria are unable to stimulate epithelial cells, but can activate 
      DC directly when DC translocate to the apical side. In conclusion, epithelial 
      cells are not simply a barrier to bacteria entering via the oral route, but 
      actively influence the activating properties of bystander DC.
FAU - Rimoldi, Monica
AU  - Rimoldi M
AD  - European Institute of Oncology, Department of Experimental Oncology, Via 
      Ripamonti 435, 20141 Milano, Italy.
FAU - Chieppa, Marcello
AU  - Chieppa M
FAU - Vulcano, Marisa
AU  - Vulcano M
FAU - Allavena, Paola
AU  - Allavena P
FAU - Rescigno, Maria
AU  - Rescigno M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Bacillus subtilis/immunology
MH  - Bacterial Infections/*immunology
MH  - Chemokines/immunology
MH  - Coculture Techniques
MH  - Cytokines/immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - Intestinal Mucosa/cytology/*immunology
MH  - Lactobacillus plantarum/immunology
MH  - Mice
MH  - Salmonella typhimurium/genetics/immunology
MH  - Tight Junctions/immunology
EDAT- 2005/02/01 09:00
MHDA- 2005/04/22 09:00
CRDT- 2005/02/01 09:00
PHST- 2005/02/01 09:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2005/02/01 09:00 [entrez]
AID - 1029/1/66 [pii]
AID - 10.1196/annals.1309.009 [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2004 Dec;1029:66-74. doi: 10.1196/annals.1309.009.