PMID- 15683652
OWN - NLM
STAT- MEDLINE
DCOM- 20050801
LR  - 20191210
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 26
IP  - 20
DP  - 2005 Jul
TI  - Scaffold fabrication by indirect three-dimensional printing.
PG  - 4281-9
AB  - Three-dimensional printing (3DP) has been employed to fabricate porous scaffolds 
      by inkjet printing liquid binder droplets onto particulate matter. Direct 3DP, 
      where the final scaffold materials are utilized during the actual 3DP process, 
      imposes several limitations on the final scaffold structure. This study describes 
      an indirect 3DP protocol, where molds are printed and the final materials are 
      cast into the mold cavity to overcome the limitations of the direct technique. To 
      evaluate the resolution available in this technique, scaffolds with villi 
      features (500 microm diameter, 1 mm height) were produced by solvent casting into 
      plaster molds, followed by particulate leaching. Scanning electron microscope 
      (SEM) showed highly open, well interconnected, uniform pore architecture ( 
      approximately 100-150 microm). The ability of these scaffolds to support 
      intestinal epithelial cell (IEC6) culture was investigated in vitro. IEC6 cells 
      attached to scaffolds uniformly in vitro and grew preferentially in the villi 
      region. To exploit the freeform nature of this technique with large pore size, 
      anatomically shaped zygoma scaffolds with 300-500 microm interconnected pores 
      were produced and characterized. Indirect 3DP provides an alternative method to 
      complement other direct solid freeform fabrication methods.
FAU - Lee, Min
AU  - Lee M
AD  - Department of Bioengineering,University of California, Los Angeles, CA 90095, 
      USA.
FAU - Dunn, James C Y
AU  - Dunn JC
FAU - Wu, Benjamin M
AU  - Wu BM
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 0 (Powders)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Biocompatible Materials/*chemistry
MH  - Cell Adhesion/physiology
MH  - Cell Culture Techniques/methods
MH  - Cell Line
MH  - Cell Proliferation
MH  - Computer-Aided Design
MH  - Intestinal Mucosa/*cytology
MH  - Lactic Acid/*chemistry
MH  - Manufactured Materials/analysis
MH  - Materials Testing
MH  - Particle Size
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
MH  - Porosity
MH  - Powders
MH  - Surface Properties
MH  - Tissue Engineering/*methods
EDAT- 2005/02/03 09:00
MHDA- 2005/08/02 09:00
CRDT- 2005/02/03 09:00
PHST- 2004/07/21 00:00 [received]
PHST- 2004/10/29 00:00 [accepted]
PHST- 2005/02/03 09:00 [pubmed]
PHST- 2005/08/02 09:00 [medline]
PHST- 2005/02/03 09:00 [entrez]
AID - S0142-9612(04)00960-3 [pii]
AID - 10.1016/j.biomaterials.2004.10.040 [doi]
PST - ppublish
SO  - Biomaterials. 2005 Jul;26(20):4281-9. doi: 10.1016/j.biomaterials.2004.10.040.