PMID- 15684769
OWN - NLM
STAT- MEDLINE
DCOM- 20050408
LR  - 20220321
IS  - 0897-7151 (Print)
IS  - 0897-7151 (Linking)
VI  - 21
IP  - 12
DP  - 2004 Dec
TI  - Manganese superoxide dismutase induced by TNF-beta is regulated transcriptionally 
      by NF-kappaB after spinal cord injury in rats.
PG  - 1778-94
AB  - Antioxidant enzymes including superoxide dismutase (SOD) may play a role in the 
      mechanism by which cells counteract the deleterious effects of reactive oxygen 
      species (ROS) after spinal cord injury (SCI). Cu/Zn and MnSOD are especially 
      potent scavengers of superoxide anion and likely serve important cytoprotective 
      roles against cellular damage. We investigated expression of SOD after SCI to 
      address its role during the early stages of injury. MnSOD activity was increased 
      4 h after SCI and persisted at elevated levels up to 24-48 h; by contrast, 
      Cu/ZnSOD activity was not changed. RT-PCR and Western blot analyses showed 
      increased levels of MnSOD mRNA and protein, respectively, by 4 h and reached 
      maximum levels by 24-48 h. Double immunostaining revealed that MnSOD protein was 
      localized within neurons and oligodendrocytes. Tumor necrosis factor-alpha 
      (TNF-alpha) was administered locally into uninjured spinal cords to examine 
      potential mechanisms for MnSOD induction after injury. TNF-alpha administered 
      exogenously increased MnSOD expression in uninjured spinal cords. Western blot 
      and immunostaining also revealed that a transcription factor, NF-kappaB, was 
      activated and translocated into the nuclei of neurons and oligodendrocytes. By 
      contrast, administration of neutralizing antibody against TNF-alpha into injured 
      spinal cords attenuated the increase in MnSOD expression and activation of 
      NF-kappaB. Double immunostaining revealed that MnSOD was co-localized with 
      NF-kappaB in neurons and oligodendrocytes after SCI. These results suggest that 
      TNF-alpha may be an inducer of NF-kappaB activation and MnSOD expression after 
      SCI and that MnSOD expression induced by TNF-alpha is likely mediated through 
      activation of NF-kappaB.
FAU - Yune, Tae Y
AU  - Yune TY
AD  - Biomedical Research Center, Korea Institute of Science & Technology, Seoul, 
      Korea. rila@kist.re.kr
FAU - Lee, Sang M
AU  - Lee SM
FAU - Kim, Sun J
AU  - Kim SJ
FAU - Park, Hong K
AU  - Park HK
FAU - Oh, Young J
AU  - Oh YJ
FAU - Kim, Young C
AU  - Kim YC
FAU - Markelonis, George J
AU  - Markelonis GJ
FAU - Oh, Tae H
AU  - Oh TH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Electrophoretic Mobility Shift Assay
MH  - Male
MH  - NF-kappa B/*physiology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spinal Cord Injuries/*enzymology
MH  - Superoxide Dismutase/genetics/*metabolism
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 2005/02/03 09:00
MHDA- 2005/04/09 09:00
CRDT- 2005/02/03 09:00
PHST- 2005/02/03 09:00 [pubmed]
PHST- 2005/04/09 09:00 [medline]
PHST- 2005/02/03 09:00 [entrez]
AID - 10.1089/neu.2004.21.1778 [doi]
PST - ppublish
SO  - J Neurotrauma. 2004 Dec;21(12):1778-94. doi: 10.1089/neu.2004.21.1778.