PMID- 15684834
OWN - NLM
STAT- MEDLINE
DCOM- 20050519
LR  - 20220223
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 13
IP  - 6
DP  - 2004 Dec
TI  - Monocytes and macrophages form branched cell columns in matrigel: implications 
      for a role in neovascularization.
PG  - 665-76
AB  - Linear arrays of cells, or cell columns, have been observed in the extracellular 
      matrix prior to neovascularization, but their nature and significance remains 
      elusive. Based on the emerging evidence implicating a role for monocytes and 
      macrophages (MC/MPH) in vasculogenesis, we hypothesized that MC/MPH also can form 
      linear or branched columns, facilitating the co-migration and the spatial 
      arrangement of other cell types. To test this hypothesis, we studied the 
      distribution of MC/MPH effected by chemotactic migration in novel in vitro and in 
      vivo models of development. We induced transversal and lateral migration of THP-1 
      monocytoid cells in Matrigel in vitro. The effect of this process on 
      co-localization of other micro-objects was assessed using erythrocytes and 
      micron-sized plastic beads. In vivo, we analyzed MC/MPH infiltration in 
      subcutaneously implanted Matrigel plugs containing angiogenic factors and across 
      a microporous filter comprising the wall of a chamber filled with Matrigel, also 
      placed subcutaneously in mice. In vitro, we found that migrating THP-1 cells 
      induced the lasting degradation of Matrigel and produced cell columns, a process 
      amplified by monocyte chemoattractant protein-1 (MCP-1). We also report the 
      co-localization of erythrocytes with THP-1 cells in cell columns. 
      Endothelium-free tunnels containing MC/MPH, neutrophils, or erythrocytes were 
      also observed in the Matrigel-filled chambers. In free subcutaneous Matrigel 
      plugs, we found MC/MPH-based columns harboring isolated Tie-2+ cells (a marker of 
      endothelial progenitor phenotype), as well as fibroblasts, dendritic cells, and 
      adypocytes. Many of these cell columns displayed conspicuous branching. Our data 
      demonstrate formation of branched MC/MPH cell columns in vitro and in vivo, a 
      previously unrecognized pattern of penetration of extracellular matrices by 
      inflammatory cells. Thus, monocytes and macrophages influence the distribution of 
      neovessels as well as their branching points. These cells are the "architects of 
      development," assisting organogenesis, tumorigenesis, and wound healing by 
      patterning the tissular space.
FAU - Anghelina, Mirela
AU  - Anghelina M
AD  - Davis Heart and Lung Research Institute, Department of Internal 
      Medicine/Cardiology, The Ohio State University, Columbus, OH 43210, USA.
FAU - Krishnan, Padma
AU  - Krishnan P
FAU - Moldovan, Leni
AU  - Moldovan L
FAU - Moldovan, Nicanor I
AU  - Moldovan NI
LA  - eng
GR  - R01 HL65983/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Chemokine CCL2)
RN  - 0 (Drug Combinations)
RN  - 0 (Laminin)
RN  - 0 (Proteoglycans)
RN  - 119978-18-6 (matrigel)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adipocytes/cytology
MH  - Animals
MH  - Cell Culture Techniques/methods
MH  - Cell Line
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemotaxis
MH  - Collagen/metabolism/*pharmacology
MH  - Dendritic Cells/cytology
MH  - Drug Combinations
MH  - Endothelium, Vascular/cytology
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Lac Operon
MH  - Laminin/metabolism/*pharmacology
MH  - Macrophages/*cytology
MH  - Mice
MH  - Mice, Transgenic
MH  - Microscopy, Phase-Contrast
MH  - Monocytes/*cytology/metabolism
MH  - *Neovascularization, Pathologic
MH  - Proteoglycans/metabolism/*pharmacology
EDAT- 2005/02/03 09:00
MHDA- 2005/05/20 09:00
CRDT- 2005/02/03 09:00
PHST- 2005/02/03 09:00 [pubmed]
PHST- 2005/05/20 09:00 [medline]
PHST- 2005/02/03 09:00 [entrez]
AID - 10.1089/scd.2004.13.665 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2004 Dec;13(6):665-76. doi: 10.1089/scd.2004.13.665.