PMID- 15686453
OWN - NLM
STAT- MEDLINE
DCOM- 20050322
LR  - 20071115
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 128
IP  - 4
DP  - 2005 Feb
TI  - Unique gene expression and clinical characteristics are associated with the 11q23 
      deletion in chronic lymphocytic leukaemia.
PG  - 460-71
AB  - Chromosome abnormalities influence prognosis and tumour progression in B-cell 
      Chronic Lymphocytic Leukaemia (CLL). This study sought to determine whether these 
      different disease subgroups were associated with unique gene expression patterns. 
      Thirty-four cases of CLL were screened for the 11q23, 13q14, 17p13 deletions, and 
      trisomy 12 by fluorescence in situ hybridization (FISH). Expression of 205 cell 
      signalling and apoptosis genes were compared by cDNA array among cases with 
      different chromosome abnormalities. A majority of the statistically 
      differentially expressed genes were present in the 11q23 deletion group by 
      hierarchical clustering. CDC2, a serine/threonine kinase, was overexpressed in 
      the 11q23 deletion group (P = 0.0004) and confirmed by Taqman real-time 
      polymerase chain reaction. Several other genes associated with cell signalling 
      were overexpressed in the 11q23 deletion group. A strong overall correlation 
      existed between the presence of different chromosome abnormalities and a number 
      of prognostic factors including immunoglobulin heavy chain variable region 
      mutation status (P = 0.011), time to treatment (P = 0.025) and lymphocyte 
      doubling time (P = 0.034). This study confirmed the prognostic impact of 
      chromosome abnormalities identified by FISH in CLL, particularly the 11q23 
      deletion and trisomy 12. In addition, the 11q23 deletion group was associated 
      with a unique gene expression pattern involving cell signalling and apoptosis 
      genes.
FAU - Dickinson, John D
AU  - Dickinson JD
AD  - Department of Genetics, Cell Biology, & Anatomy, University of Nebraska Medical 
      Center, Omaha, NE 68198-6395, USA.
FAU - Smith, Lynette M
AU  - Smith LM
FAU - Sanger, Warren G
AU  - Sanger WG
FAU - Zhou, Guimei
AU  - Zhou G
FAU - Townley, Peter
AU  - Townley P
FAU - Lynch, James C
AU  - Lynch JC
FAU - Pavletic, Z Steven
AU  - Pavletic ZS
FAU - Bierman, Philip J
AU  - Bierman PJ
FAU - Joshi, Shantaram S
AU  - Joshi SS
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin Variable Region)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Apoptosis/genetics
MH  - Chromosome Aberrations
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunoglobulin Variable Region/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - Prognosis
MH  - Signal Transduction/genetics
EDAT- 2005/02/03 09:00
MHDA- 2005/03/23 09:00
CRDT- 2005/02/03 09:00
PHST- 2005/02/03 09:00 [pubmed]
PHST- 2005/03/23 09:00 [medline]
PHST- 2005/02/03 09:00 [entrez]
AID - BJH5344 [pii]
AID - 10.1111/j.1365-2141.2004.05344.x [doi]
PST - ppublish
SO  - Br J Haematol. 2005 Feb;128(4):460-71. doi: 10.1111/j.1365-2141.2004.05344.x.