PMID- 15686468
OWN - NLM
STAT- MEDLINE
DCOM- 20050322
LR  - 20071114
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 128
IP  - 4
DP  - 2005 Feb
TI  - The effect of hypoxia and stem cell source on haemoglobin switching.
PG  - 562-70
AB  - This study investigated whether relative changes that accompany the naturally 
      occurring shifts in haematopoietic sites during human development play a role in 
      haemoglobin (Hb) switching or whether Hb switching is innately programmed into 
      cells. CD34(+)/Lineage(-) haematopoietic stem/progenitor cells (HSCs) were 
      isolated from human fetal liver (F-LVR), cord blood (CB), and adult bone marrow 
      (ABM), and the Hb was characterized by flow cytometry on cultures that generated 
      enucleated red cells. All feeder layers (stroma from F-LVR, ABM, and human fetal 
      aorta) enhanced cell proliferation and erythropoiesis but did not affect Hb type. 
      HSCs from CB and F-LVR generated the same Hb profile under normoxia and hypoxia. 
      HSCs from ABM had single-positive HbA and double-positive HbA and HbF cells at 
      normoxia and almost entirely double-positive cells at hypoxia. Further 
      characterization of these ABM cultures was determined by following mRNA 
      expression for the transcription factors erythroid Kruppel-like factor (EKLF) and 
      fetal Kruppel-like factor (FKLF) as a function of time in cultures under hypoxia 
      and normoxia. The erythroid-specific isoform of 5-amino-levulinate synthase 
      (ALAS2) was also expressed under hypoxic conditions. We conclude that Hb 
      switching is affected by the environment but not all HSCs are preprogrammed to 
      respond.
FAU - Narayan, A Daisy
AU  - Narayan AD
AD  - Department of Animal Biotechnology, University of Nevada, Reno, NV 89557, USA. 
      daisy@med.unr.edu
FAU - Ersek, Adel
AU  - Ersek A
FAU - Campbell, Thomas A
AU  - Campbell TA
FAU - Colon, Donna M
AU  - Colon DM
FAU - Pixley, John S
AU  - Pixley JS
FAU - Zanjani, Esmail D
AU  - Zanjani ED
LA  - eng
GR  - HL66058-02/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Culture Media, Serum-Free)
RN  - 9034-51-9 (Hemoglobin A)
RN  - 9034-63-3 (Fetal Hemoglobin)
SB  - IM
MH  - Adult
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/physiology
MH  - Cell Division/physiology
MH  - Cell Hypoxia/physiology
MH  - Cell Lineage/physiology
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free
MH  - Erythropoiesis/*physiology
MH  - Fetal Blood/cytology
MH  - Fetal Hemoglobin/*metabolism
MH  - Hematopoietic Stem Cells/*cytology
MH  - Hemoglobin A/metabolism
MH  - Humans
MH  - Liver/cytology/embryology
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2005/02/03 09:00
MHDA- 2005/03/23 09:00
CRDT- 2005/02/03 09:00
PHST- 2005/02/03 09:00 [pubmed]
PHST- 2005/03/23 09:00 [medline]
PHST- 2005/02/03 09:00 [entrez]
AID - BJH5336 [pii]
AID - 10.1111/j.1365-2141.2004.05336.x [doi]
PST - ppublish
SO  - Br J Haematol. 2005 Feb;128(4):562-70. doi: 10.1111/j.1365-2141.2004.05336.x.