PMID- 15688635
OWN - NLM
STAT- MEDLINE
DCOM- 20050307
LR  - 20061115
IS  - 1615-9306 (Print)
IS  - 1615-9306 (Linking)
VI  - 28
IP  - 1
DP  - 2005 Jan
TI  - Protocol of capillary isoelectric focusing to separate extremely acidic and basic 
      proteins.
PG  - 78-86
AB  - A new set-up was constructed for capillary isoelectric focusing (CIEF) involving 
      a sampling capillary as a bypass fixed to the separation capillary. Sample 
      solutions were subjected to a previously established pH gradient from the sample 
      capillary. Besides performing conventional CIEF, the separation of ampholytic 
      compounds with isoelectric points (p/s) beyond the pH gradient was carried out on 
      this system. This method was termed as pH gradient driven electrophoresis (PGDE) 
      and the basic mathematical expressions were derived to express the dynamic 
      fundamentals. Proteins such as lysozyme, cytochrome C, and pepsin with p/s higher 
      than 10 or below 3 were separated in a pH gradient provided by Pharmalyte (pH 
      3-10). Finally, this protocol convincingly exhibited its potential in the 
      separation of a solution of chicken egg white.
FAU - Yang, Chun
AU  - Yang C
AD  - Department of Biotechnology, National Chromatographic R & A Center, Dalian 
      Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.
FAU - Zhang, Weibing
AU  - Zhang W
FAU - Zhang, Jie
AU  - Zhang J
FAU - Duan, Jicheng
AU  - Duan J
FAU - Zhang, Yukui
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Proteins)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 3.2.1.17 (Muramidase)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Animals
MH  - Biometry
MH  - Cytochromes c/chemistry/isolation & purification
MH  - Electrophoresis, Capillary/instrumentation/*methods/statistics & numerical data
MH  - Hydrogen-Ion Concentration
MH  - Isoelectric Focusing/instrumentation/*methods/statistics & numerical data
MH  - Muramidase/chemistry/isolation & purification
MH  - Pepsin A/chemistry/isolation & purification
MH  - Proteins/chemistry/*isolation & purification
EDAT- 2005/02/04 09:00
MHDA- 2005/03/08 09:00
CRDT- 2005/02/04 09:00
PHST- 2005/02/04 09:00 [pubmed]
PHST- 2005/03/08 09:00 [medline]
PHST- 2005/02/04 09:00 [entrez]
AID - 10.1002/jssc.200401793 [doi]
PST - ppublish
SO  - J Sep Sci. 2005 Jan;28(1):78-86. doi: 10.1002/jssc.200401793.