PMID- 15690798
OWN - NLM
STAT- MEDLINE
DCOM- 20050310
LR  - 20230307
IS  - 0362-028X (Print)
IS  - 0362-028X (Linking)
VI  - 68
IP  - 1
DP  - 2005 Jan
TI  - Isolation of Bacillus circulans and Paenibacillus polymyxa strains inhibitory to 
      Campylobacter jejuni and characterization of associated bacteriocins.
PG  - 11-7
AB  - We evaluated anti-Campylobacter activity among 365 Bacillus and Paenibacillus 
      isolates from poultry production environments. One novel antagonistic Bacillus 
      circulans and three Paenibacillus polymyxa strains were identified and further 
      studied. Cell-free ammonium sulfate precipitate (crude antimicrobial preparation) 
      was obtained from each candidate culture. Zones of Campylobacter growth 
      inhibition surrounding 10 microl of this crude antimicrobial preparation were 
      quantified using a spot test. Campylobacter growth resumed when the preparation 
      was preincubated with selected protease enzymes, demonstrating peptide 
      characteristics consistent with a bacteriocin. These peptides were further 
      purified using combinations of molecular mass resolution and ion exchange 
      chromatography. Molecular masses of the peptides were estimated at approximately 
      3,500 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 
      Isoelectric focusing was used to determine the pI values of the peptides. Amino 
      acid sequences of the bacteriocins and more precise molecular masses were 
      obtained by matrix-assisted laser desorption and ionization-time of flight 
      (MALDI-TOF) analysis. The bacteriocin from P. polymyxa NRRL B-30507 had a pI of 
      4.8, that from P. polymyxa NRRL B-30509 had a pI of 7.2, that from P. polymyxa 
      NRRL B-30508 had a pI of 4.8, and that from B. circulans NRRL B-30644 had a pI of 
      7.8. The amino acid sequences were consistent with those of class IIa 
      bacteriocins. These antagonists and the corresponding bacteriocins may be useful 
      in the control of Campylobacter infection in poultry.
FAU - Svetoch, Edward A
AU  - Svetoch EA
AD  - State Research Center for Applied Microbiology, Obolensk, Russian Federation.
FAU - Stern, Norman J
AU  - Stern NJ
FAU - Eruslanov, Boris V
AU  - Eruslanov BV
FAU - Kovalev, Yuri N
AU  - Kovalev YN
FAU - Volodina, Larisa I
AU  - Volodina LI
FAU - Perelygin, Vladimir V
AU  - Perelygin VV
FAU - Mitsevich, Evgeni V
AU  - Mitsevich EV
FAU - Mitsevich, Irina P
AU  - Mitsevich IP
FAU - Pokhilenko, Victor D
AU  - Pokhilenko VD
FAU - Borzenkov, Valery N
AU  - Borzenkov VN
FAU - Levchuk, Vladimir P
AU  - Levchuk VP
FAU - Svetoch, Olga E
AU  - Svetoch OE
FAU - Kudriavtseva, Tamara Y
AU  - Kudriavtseva TY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacteriocins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacillus/isolation & purification/*physiology
MH  - Bacterial Proteins/isolation & purification/metabolism
MH  - Bacteriocins/*isolation & purification/metabolism
MH  - Campylobacter Infections/prevention & control/*veterinary
MH  - Campylobacter jejuni/*growth & development/metabolism
MH  - Colony Count, Microbial
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Isoelectric Focusing
MH  - Molecular Weight
MH  - Poultry
MH  - Poultry Diseases/*prevention & control
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2005/02/05 09:00
MHDA- 2005/03/11 09:00
CRDT- 2005/02/05 09:00
PHST- 2005/02/05 09:00 [pubmed]
PHST- 2005/03/11 09:00 [medline]
PHST- 2005/02/05 09:00 [entrez]
AID - S0362-028X(22)00876-6 [pii]
AID - 10.4315/0362-028x-68.1.11 [doi]
PST - ppublish
SO  - J Food Prot. 2005 Jan;68(1):11-7. doi: 10.4315/0362-028x-68.1.11.