PMID- 15691323
OWN - NLM
STAT- MEDLINE
DCOM- 20050328
LR  - 20091119
IS  - 1742-464X (Print)
IS  - 1742-464X (Linking)
VI  - 272
IP  - 4
DP  - 2005 Feb
TI  - A 'pure' chemoattractant formylpeptide analogue triggers a specific signalling 
      pathway in human neutrophil chemotaxis.
PG  - 883-91
AB  - As it has not yet been established whether the second messengers involved in the 
      neutrophil response have identical or specific signalling requirements for each 
      physiological function, protein kinase C (PKC) isoforms and mitogen activated 
      protein kinases (MAPKs) were studied in human chemotaxis triggered by the full 
      agonist for-Met-Leu-Phe-OMe (fMLP-OMe) and the 'pure' chemoattractant 
      for-Thp-Leu-Ain-OMe [Thp1,Ain3] analogue. Experiments were performed in the 
      presence or absence of extracellular Ca2+, known to be an important modulator of 
      second messengers. Our data demonstrate that specific PKC beta1 translocation and 
      p38 MAPK phosphorylation are strongly associated with the chemotactic response of 
      the neutrophils triggered by both peptides, while Ca2+ is not necessary for 
      chemotaxis to occur. PKC and MAPK inhibitors were used in Western blotting assays 
      and in cell locomotion experiments to investigate if the MAPK signalling pathway 
      was controlled by PKC activation. The most important finding emerging from this 
      study is that PKC and MAPK activate the chemotactic function of human neutrophils 
      by two independent pathways.
FAU - Spisani, Susanna
AU  - Spisani S
AD  - Dipartimento di Biochimica e Biologia Molecolare, Universita degli Studi di 
      Ferrara, Italy.
FAU - Falzarano, Sofia
AU  - Falzarano S
FAU - Traniello, Serena
AU  - Traniello S
FAU - Nalli, Marianna
AU  - Nalli M
FAU - Selvatici, Rita
AU  - Selvatici R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS J
JT  - The FEBS journal
JID - 101229646
RN  - 0 (Chemotactic Factors)
RN  - 0 (Peptides)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Blotting, Western
MH  - Chemotactic Factors/*metabolism
MH  - Chemotaxis/*physiology
MH  - Humans
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors
MH  - Neutrophils/*metabolism
MH  - Peptides/*metabolism
MH  - Protein Kinase C/antagonists & inhibitors
MH  - Signal Transduction/*physiology
EDAT- 2005/02/05 09:00
MHDA- 2005/03/29 09:00
CRDT- 2005/02/05 09:00
PHST- 2005/02/05 09:00 [pubmed]
PHST- 2005/03/29 09:00 [medline]
PHST- 2005/02/05 09:00 [entrez]
AID - EJB4497 [pii]
AID - 10.1111/j.1742-4658.2004.04497.x [doi]
PST - ppublish
SO  - FEBS J. 2005 Feb;272(4):883-91. doi: 10.1111/j.1742-4658.2004.04497.x.