PMID- 15693092
OWN - NLM
STAT- MEDLINE
DCOM- 20050428
LR  - 20220408
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 32
IP  - 2
DP  - 2005 Feb
TI  - Fibroblast-like synovial cells derived from synovial fluid.
PG  - 301-6
AB  - OBJECTIVE: To obtain fibroblast-like synovial cells (FLS) from synovial fluid 
      (SF). METHODS: SF aspirated from joints of patients with rheumatoid arthritis 
      (RA), other types of inflammatory arthritis, and osteoarthritis (OA) was 
      centrifuged and the resulting cell pellet resuspended in growth medium. After 2 
      days, nonadherent cells were removed. FLS were also cultured from surgical 
      specimens of synovial tissue (td-FLS). Phenotype characterization of fluid 
      derived FLS (fd-FLS) was accomplished by flow cytometry and immunohistochemistry 
      staining. Tumor necrosis factor-alpha (TNF-alpha) induced interleukin 6 (IL-6), 
      IL-8, and cyclooxygenase 2 (COX-2) mRNA levels were assessed. RESULTS: Second and 
      later passage fd-FLS exhibited uniform fibroblast-like morphology. Fd-FLS and 
      td-FLS expressed a similar profile of cell surface antigens including the 
      fibroblast marker Thy-1. Less than 2% of either cell type expressed surface 
      markers characteristic of dendritic cells, phagocytic cells, T cells, or 
      leukocytes. Immunohistochemistry staining revealed the presence of fibroblast 
      products prolyl-4 hydroxylase, procollagen I, and procollagen III in both culture 
      types. TNF-a induced increases in IL-6, IL-8, and COX-2 mRNA were suppressed by 
      dexamethasone in both fd-FLS and td-FLS. CONCLUSION: FLS can be cultured from SF. 
      The fibroblast phenotype was confirmed by analysis of surface antigens and 
      intracellular proteins. Inflammatory mediators produced after stimulation of both 
      fd-FLS and td-FLS were suppressed by dexamethasone. In addition to providing a 
      more accessible source of FLS, fd-FLS may also facilitate study of synovial cells 
      in early RA when tissue specimens are not readily available.
FAU - Stebulis, Judith A
AU  - Stebulis JA
AD  - Department of Medicine, Rheumatology Division, University of Massachusetts 
      Medical School, Worcester, Massachusetts, USA. judith.stebulis@umassmed.edu
FAU - Rossetti, Ronald G
AU  - Rossetti RG
FAU - Atez, Francisco J
AU  - Atez FJ
FAU - Zurier, Robert B
AU  - Zurier RB
LA  - eng
GR  - DK32520/DK/NIDDK NIH HHS/United States
GR  - R01 AR3850/AR/NIAMS NIH HHS/United States
GR  - R01 DA13691/DA/NIDA NIH HHS/United States
GR  - R21 AT001471/AT/NCCIH NIH HHS/United States
GR  - T32 AR07572/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
SB  - IM
MH  - Arthritis/metabolism/*pathology
MH  - Arthritis, Rheumatoid/metabolism/pathology
MH  - Cells, Cultured
MH  - Cyclooxygenase 2
MH  - Dexamethasone/pharmacology
MH  - Fibroblasts/drug effects/metabolism/*pathology
MH  - Flow Cytometry
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interleukin-6/genetics/metabolism
MH  - Interleukin-8/genetics/metabolism
MH  - Knee Joint/*pathology
MH  - Membrane Proteins
MH  - Osteoarthritis, Knee/metabolism/pathology
MH  - Phenotype
MH  - Prostaglandin-Endoperoxide Synthases/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - *Synovial Fluid
MH  - Synovial Membrane/metabolism/*pathology
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
EDAT- 2005/02/05 09:00
MHDA- 2005/04/29 09:00
CRDT- 2005/02/05 09:00
PHST- 2005/02/05 09:00 [pubmed]
PHST- 2005/04/29 09:00 [medline]
PHST- 2005/02/05 09:00 [entrez]
AID - 0315162X-32-301 [pii]
PST - ppublish
SO  - J Rheumatol. 2005 Feb;32(2):301-6.