PMID- 15695515
OWN - NLM
STAT- MEDLINE
DCOM- 20050621
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 280
IP  - 14
DP  - 2005 Apr 8
TI  - Interactions of multiple heparin binding growth factors with neuropilin-1 and 
      potentiation of the activity of fibroblast growth factor-2.
PG  - 13457-64
AB  - The hypothesis that neuropilin-1 (Npn-1) may interact with heparin-binding 
      proteins other than vascular endothelial growth factor has been tested using an 
      optical biosensor-based binding assay. The results show that fibroblast growth 
      factor (FGF) 1, 2, 4, and 7, FGF receptor 1, hepatocyte growth factor/scatter 
      factor (HGF/SF), FGF-binding protein, normal protease sensitive form of prion 
      protein, antithrombin III, and Npn-1 itself are all able to interact with Npn-1 
      immobilized on the sensor surface. FGF-2, FGF-4, and HGF/SF are also shown to 
      interact with Npn-1 in a solution assay. Moreover, these protein-protein 
      interactions are dependent on the ionic strength of the medium and are inhibited 
      by heparin, and the kinetics of binding of FGF-2, FGF-4 and HGF/SF to Npn-1 are 
      characterized by fast association rate constants (270,000-1,600,000 m(-1) s(-1)). 
      These results suggest that Npn-1 possesses a "heparin" mimetic site that is able 
      to interact at least in part through ionic bonding with the heparin binding site 
      on many of the proteins studied. Npn-1 was also found to potentiate the growth 
      stimulatory activity of FGF-2 on human umbilical vein endothelial cells, 
      indicating that Npn-1 may not just bind but also regulate the activity of 
      heparin-binding proteins.
FAU - West, David C
AU  - West DC
AD  - School of Biological Sciences, Biosciences Building, University of Liverpool, 
      Liverpool, L69 7ZB, United Kingdom.
FAU - Rees, Chris G
AU  - Rees CG
FAU - Duchesne, Laurence
AU  - Duchesne L
FAU - Patey, Susannah J
AU  - Patey SJ
FAU - Terry, Carla J
AU  - Terry CJ
FAU - Turnbull, Jeremy E
AU  - Turnbull JE
FAU - Delehedde, Maryse
AU  - Delehedde M
FAU - Heegaard, Christian W
AU  - Heegaard CW
FAU - Allain, Fabrice
AU  - Allain F
FAU - Vanpouille, Christophe
AU  - Vanpouille C
FAU - Ron, Dina
AU  - Ron D
FAU - Fernig, David G
AU  - Fernig DG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050204
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Polysaccharides)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 144713-63-3 (Neuropilin-1)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9005-49-6 (Heparin)
RN  - EC 2.7.10.1 (FGFR1 protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Biosensing Techniques
MH  - Cell Line
MH  - Endothelial Cells/cytology/metabolism
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Heparin/*metabolism
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Models, Molecular
MH  - Neuropilin-1/chemistry/*metabolism
MH  - Polysaccharides/metabolism
MH  - Protein Isoforms/metabolism
MH  - Protein Structure, Tertiary
MH  - Receptor Protein-Tyrosine Kinases/metabolism
MH  - Receptor, Fibroblast Growth Factor, Type 1
MH  - Receptors, Fibroblast Growth Factor/metabolism
EDAT- 2005/02/08 09:00
MHDA- 2005/06/23 09:00
CRDT- 2005/02/08 09:00
PHST- 2005/02/08 09:00 [pubmed]
PHST- 2005/06/23 09:00 [medline]
PHST- 2005/02/08 09:00 [entrez]
AID - S0021-9258(19)60414-X [pii]
AID - 10.1074/jbc.M410924200 [doi]
PST - ppublish
SO  - J Biol Chem. 2005 Apr 8;280(14):13457-64. doi: 10.1074/jbc.M410924200. Epub 2005 
      Feb 4.