PMID- 15698414
OWN - NLM
STAT- MEDLINE
DCOM- 20050526
LR  - 20131121
IS  - 1478-3223 (Print)
IS  - 1478-3223 (Linking)
VI  - 25
IP  - 1
DP  - 2005 Feb
TI  - Normal liver regeneration and liver cell apoptosis after partial hepatectomy in 
      tumor necrosis factor-alpha-deficient mice.
PG  - 162-70
AB  - AIMS/BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is known as a 
      proinflammatory cytokine that has been implicated as a contributing factor in a 
      number of disease processes. TNF-alpha also influences liver repair following 
      hepatotoxic damage, and regeneration following partial hepatectomy (PH). The aim 
      of this study was to assess the mechanism by which TNF-alpha influences liver 
      cell apoptosis and regeneration following PH in TNF-alpha-deficient 
      (TNF-alpha(-/-)) mice. METHODS: PH was performed in wild mice and TNF-alpha(-/-) 
      mice. RESULTS: In both groups, serum alanine aminotransferase and serum total 
      bilirubin levels comparably peaked at 6 and 48 h after PH, respectively. No 
      differences were observed in hepatocyte proliferation, as determined by mitotic 
      and the proliferating cell nuclear antigen labeling indices, between 
      TNF-alpha(+/+) and TNF-alpha(-/-) mice. Few terminal deoxynucleotidyl transferase 
      nick end-labeling-positive hepatocytes were seen in either type of mice. Nuclear 
      factor-kappa B DNA binding activity in the remaining liver of TNF-alpha(-/-) mice 
      after PH was similar to that of control mice. Ribonuclease protection assay 
      showed that transforming growth factor beta1 mRNA was up-regulated comparably in 
      the livers of the two groups, and that other cytokines were hardly seen in 
      either. Interleukin-6/ signal transducer and activator of 
      transcription-3-dependent pathway was not affected in TNF-alpha(-/-) mice. 
      CONCLUSIONS: These findings suggest that TNF-alpha has little influence on liver 
      regeneration and liver cell apoptosis after PH in mice.
FAU - Hayashi, Hideki
AU  - Hayashi H
AD  - First Department of Internal Medicine, Gifu University School of Medicine, Gifu 
      501-1194, Japan.
FAU - Nagaki, Masahito
AU  - Nagaki M
FAU - Imose, Motoaki
AU  - Imose M
FAU - Osawa, Yosuke
AU  - Osawa Y
FAU - Kimura, Kiminori
AU  - Kimura K
FAU - Takai, Shinji
AU  - Takai S
FAU - Imao, Motohiro
AU  - Imao M
FAU - Naiki, Takafumi
AU  - Naiki T
FAU - Kato, Tomohiro
AU  - Kato T
FAU - Moriwaki, Hisataka
AU  - Moriwaki H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - RFM9X3LJ49 (Bilirubin)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Bilirubin/blood
MH  - *Hepatectomy
MH  - Hepatocytes/metabolism/*pathology
MH  - In Situ Nick-End Labeling
MH  - Liver Regeneration/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mitotic Index
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - RNA, Messenger/metabolism
MH  - Transforming Growth Factor beta/genetics/metabolism
MH  - Transforming Growth Factor beta1
MH  - Tumor Necrosis Factor-alpha/*deficiency/genetics
MH  - Up-Regulation
EDAT- 2005/02/09 09:00
MHDA- 2005/05/27 09:00
CRDT- 2005/02/09 09:00
PHST- 2005/02/09 09:00 [pubmed]
PHST- 2005/05/27 09:00 [medline]
PHST- 2005/02/09 09:00 [entrez]
AID - LIV1029 [pii]
AID - 10.1111/j.1478-3231.2005.01029.x [doi]
PST - ppublish
SO  - Liver Int. 2005 Feb;25(1):162-70. doi: 10.1111/j.1478-3231.2005.01029.x.