PMID- 15699583
OWN - NLM
STAT- MEDLINE
DCOM- 20050808
LR  - 20190608
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 51
IP  - 2
DP  - 2005 Apr
TI  - Immunolocalization of steroidogenic enzymes in the corpus luteum and placenta of 
      the Japanese Shiba goat.
PG  - 247-52
AB  - In this study, we performed immunohistochemistry of cholesterol side-chain 
      cleavage cytochrome P450 (P450scc), 3beta-hydroxysteroid dehydrogenase 
      (3betaHSD), cytochrome 17alpha-hydroxylase P450 (P450c17), and cytochrome P450 
      aromatase (P450arom) in the corpus luteum and placenta of Shiba goats. The aim 
      was to clarify the steroidogenic capability of the corpus luteum and placenta of 
      Shiba goats. Ovaries containing corpora lutea were obtained from four adult Shiba 
      goats during the luteal phase (day10; n=2) and pregnancy (90 and 120 days of 
      gestation). Placenta was obtained from one Shiba goat on day 120 of gestation. 
      The sections of the ovaries and placentae were immunostained using the 
      avidin-biotin-peroxidase complex method (ABC) with polyclonal antibodies 
      generated against steroidogenic enzymes of mammalian origin. All luteal cells 
      expressed P450scc, 3betaHSD, P450c17 and P450arom. The distribution of P450scc, 
      3betaHSD, P450c17 and P450arom were not different during the luteal phase and 
      pregnancy. P450arom showed a weak positive staining in late pregnancy (120 days). 
      In addition, immunoreactions for P450c17 and P450arom were observed in 
      syncytiotrophoblast of the placenta of one Shiba goat. These results indicate 
      that, in Shiba goats, corpus luteum is not only an important source of 
      progesterone but also has the ability to synthesize androgen and estrogen during 
      the luteal phase and pregnancy. Also the placenta has the ability to synthesize 
      androgen and estrogen in late pregnancy.
FAU - Weng, Qiang
AU  - Weng Q
AD  - Faculty of Biological Science and Technology, BeiJing Forestry University, China.
FAU - Medan, Mohamed S
AU  - Medan MS
FAU - Ren, LongQuan
AU  - Ren L
FAU - Watanabe, Gen
AU  - Watanabe G
FAU - Tsubota, Toshio
AU  - Tsubota T
FAU - Taya, Kazuyoshi
AU  - Taya K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050207
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.51 (3 (or 17)-beta-hydroxysteroid dehydrogenase)
RN  - EC 1.14.- (Steroid Hydroxylases)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/metabolism
MH  - 3-Hydroxysteroid Dehydrogenases/metabolism
MH  - Animals
MH  - Aromatase/metabolism
MH  - Cholesterol Side-Chain Cleavage Enzyme/metabolism
MH  - Corpus Luteum/*enzymology
MH  - Female
MH  - Goats/*physiology
MH  - Immunohistochemistry
MH  - Luteal Phase/physiology
MH  - Placenta/*enzymology
MH  - Pregnancy
MH  - Progesterone/metabolism
MH  - Steroid Hydroxylases/*metabolism
EDAT- 2005/02/09 09:00
MHDA- 2005/08/09 09:00
CRDT- 2005/02/09 09:00
PHST- 2005/02/09 09:00 [pubmed]
PHST- 2005/08/09 09:00 [medline]
PHST- 2005/02/09 09:00 [entrez]
AID - JST.JSTAGE/jrd/16081 [pii]
AID - 10.1262/jrd.16081 [doi]
PST - ppublish
SO  - J Reprod Dev. 2005 Apr;51(2):247-52. doi: 10.1262/jrd.16081. Epub 2005 Feb 7.