PMID- 15703262
OWN - NLM
STAT- MEDLINE
DCOM- 20050801
LR  - 20161124
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 85
IP  - 1
DP  - 2005 May
TI  - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential 
      gene expression in male Japanese medaka (Oryzias latipes).
PG  - 572-84
AB  - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a ubiquitous environmental 
      contaminant with well-known adverse effects in fish. In this study, we initially 
      exploited suppression subtractive hybridization (SSH) as a screening tool to 
      assess qualitative gene expression changes in whole brain, liver, and testis of 
      adult male Japanese medaka (Oryzias latipes) exposed for 48 h to a single 
      intraperitoneal-injected dose of TCDD (10 microg TCDD/kg body weight). Across 
      these three organs, SSH identified a total of 335 unique genes. Each set of 
      forward- and reverse-subtracted organ cDNA libraries consisted of a distinct gene 
      list and corresponding distribution of biological processes, suggesting that 
      transcript profiles of these libraries were highly organ-specific. Based on 
      sequence match significance and frequencies within each set of organ libraries, 
      genes hypothesized to be strongly responsive (42 total) within male medaka brain, 
      liver, or testis were semi-quantitatively screened with replicate cDNA nylon 
      membrane arrays. In addition, TCDD-treated male medaka were surveyed for gross 
      histological analysis of brain, liver, and testis. In general, adverse 
      histopathological changes were not observed in the brain, and glycogen depletion 
      was observed only in the liver. However, significant histological changes 
      occurred in the testis, and included disorganization of spermatogenesis at the 
      testis periphery, disruption of the interstitium, Leydig cell swelling, and 
      Sertoli cell vacuolation. Of the 42 genes screened by cDNA array analysis, 
      cytochrome P450 1A (CYP1A) mRNA was the only transcript significantly higher in 
      TCDD-exposed brain, whereas 12 transcripts (including CYP1A) were significantly 
      higher in TCDD-exposed liver, and 34 transcripts were significantly lower in 
      TCDD-exposed testis. Therefore, the degree of TCDD-induced alterations observed 
      in each organ at a gross histological level corresponded well with the number and 
      ontology of gene transcripts affected on the array. Based on real-time reverse 
      transcription polymerase chain reaction (RT-PCR), relative CYP1A (but not AHR1) 
      transcript levels were confirmed to be significantly higher in TCDD-treated brain 
      and liver. However, CYP1A was not significantly induced in TCDD-exposed testis, 
      suggesting that gene expression and histopathological responses observed in the 
      testis at 48 h may be CYP1A-independent. Based on these data, unique 
      liver-specific and testis-specific mRNA-level targets in male medaka were 
      identified as promising biomarkers of acute TCDD-induced toxicity.
FAU - Volz, David C
AU  - Volz DC
AD  - Integrated Toxicology Program and Nicholas School of the Environment and Earth 
      Sciences, Duke University, Durham, North Carolina 27708-0328, USA.
FAU - Bencic, David C
AU  - Bencic DC
FAU - Hinton, David E
AU  - Hinton DE
FAU - Law, J McHugh
AU  - Law JM
FAU - Kullman, Seth W
AU  - Kullman SW
LA  - eng
GR  - 1R01 RR 018583/RR/NCRR NIH HHS/United States
GR  - 5P42 ES0004699/ES/NIEHS NIH HHS/United States
GR  - P30 ES03828/ES/NIEHS NIH HHS/United States
GR  - T32 ES07031/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050209
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polychlorinated Dibenzodioxins)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/metabolism/pathology
MH  - Environmental Pollutants/*toxicity
MH  - Gene Expression/*drug effects
MH  - Gene Expression Profiling
MH  - Injections, Intraperitoneal
MH  - Liver/*drug effects/metabolism/pathology
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - Organ Specificity
MH  - Oryzias/*genetics
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Testis/*drug effects/metabolism/pathology
EDAT- 2005/02/11 09:00
MHDA- 2005/08/02 09:00
CRDT- 2005/02/11 09:00
PHST- 2005/02/11 09:00 [pubmed]
PHST- 2005/08/02 09:00 [medline]
PHST- 2005/02/11 09:00 [entrez]
AID - kfi109 [pii]
AID - 10.1093/toxsci/kfi109 [doi]
PST - ppublish
SO  - Toxicol Sci. 2005 May;85(1):572-84. doi: 10.1093/toxsci/kfi109. Epub 2005 Feb 9.