PMID- 15703445
OWN - NLM
STAT- MEDLINE
DCOM- 20050809
LR  - 20210217
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 4
IP  - 5
DP  - 2005 May
TI  - Intact-protein-based high-resolution three-dimensional quantitative analysis 
      system for proteome profiling of biological fluids.
PG  - 618-25
AB  - The substantial complexity and vast dynamic range of protein abundance in 
      biological fluids, notably serum and plasma, present a formidable challenge for 
      comprehensive protein analysis. Integration of multiple technologies is required 
      to achieve high-resolution and high-sensitivity proteomics analysis of biological 
      fluids. We have implemented an orthogonal three-dimensional intact-protein 
      analysis system (IPAS), coupled with protein tagging and immunodepletion of 
      abundant proteins, to quantitatively profile the human plasma proteome. Following 
      immunodepletion, plasma proteins in each of paired samples are concentrated and 
      labeled with a different Cy dye, before mixing. Proteins are subsequently 
      separated in three dimensions according to their charge, hydrophobicity, and 
      molecular mass. Differences in the abundance of resolved proteins are determined 
      based on Cy dye ratios. We have applied this strategy to profile the plasma 
      proteome for changes that occur with acute graft-versus-host disease (GVHD), 
      following allogeneic bone marrow transplantation (BMT). Using capillary HPLC ESI 
      Q-TOF MS, we identified 75 proteins in the micromolar to femtomolar range that 
      exhibited quantitative differences between the pre- and post-GVHD samples. These 
      proteins included serum amyloid A, apolipoproteins A-I/A-IV, and complement C3 
      that are well-known acute-phase reactants likely reflecting the post-BMT 
      inflammatory state. In addition, we identified some potentially interesting 
      immunologically relevant molecules including vitamin D-binding protein, fetuin, 
      vitronectin, proline-rich protein 3 and 4, integrin-alpha, and leukocyte antigen 
      CD97. IPAS provides a combination of comprehensive profiling and quantitative 
      analysis, with a substantial dynamic range, for disease-related applications.
FAU - Wang, Hong
AU  - Wang H
AD  - Department of Pediatrics, University of Michigan, Ann Arbor, Michigan 48109, USA.
FAU - Clouthier, Shawn G
AU  - Clouthier SG
FAU - Galchev, Vladimir
AU  - Galchev V
FAU - Misek, David E
AU  - Misek DE
FAU - Duffner, Ulrich
AU  - Duffner U
FAU - Min, Chang-Ki
AU  - Min CK
FAU - Zhao, Rong
AU  - Zhao R
FAU - Tra, John
AU  - Tra J
FAU - Omenn, Gilbert S
AU  - Omenn GS
FAU - Ferrara, James L M
AU  - Ferrara JL
FAU - Hanash, Samir M
AU  - Hanash SM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20050209
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
RN  - 0 (Blood Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Adult
MH  - Antibody Affinity
MH  - Blood Proteins/*analysis/chemistry/immunology
MH  - *Bone Marrow Transplantation
MH  - Chromatography, Affinity/*methods
MH  - Chromatography, High Pressure Liquid
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Graft vs Host Disease/*blood/therapy
MH  - Humans
MH  - Plasma/chemistry/immunology
MH  - Proteome/*analysis/immunology
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - *Transplantation Conditioning
EDAT- 2005/02/11 09:00
MHDA- 2005/08/10 09:00
CRDT- 2005/02/11 09:00
PHST- 2005/02/11 09:00 [pubmed]
PHST- 2005/08/10 09:00 [medline]
PHST- 2005/02/11 09:00 [entrez]
AID - S1535-9476(20)31487-0 [pii]
AID - 10.1074/mcp.M400126-MCP200 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2005 May;4(5):618-25. doi: 10.1074/mcp.M400126-MCP200. Epub 
      2005 Feb 9.