PMID- 15703839
OWN - NLM
STAT- MEDLINE
DCOM- 20050617
LR  - 20161124
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 26
IP  - 3
DP  - 2005 Mar
TI  - Phosphorylation of mdm2 at serine 269 impairs its interaction with the 
      retinoblastoma protein.
PG  - 801-8
AB  - We previously reported that protein kinase CK2 phosphorylates the human mdm2 
      (hdm2) protein at serine residue 269. This phosphorylation site is located in the 
      central acidic, highly-conserved region of mdm2, which is responsible for the 
      interaction with a number of proteins. Studying the influence of phosphorylation 
      of mdm2 by CK2 upon interaction with some of these binding partners, we found 
      that the retinoblastoma (Rb) protein bound more strongly to the unphosphorylated 
      mdm2 than to its CK2-phosphorylated counterpart. An S269 phosphospecific antibody 
      was generated, and reacted with a 60 kDa subpopulation of mdm2 in human cells. We 
      created a mutant mdm2 with a serine to aspartic acid exchange at position 269, 
      which was used to transfect mdm2-/- cells. Cells transfected with the S269D 
      mutant exhibited a different growth behavior than wild-type mdm2-expressing 
      cells, which might be attributed to the altered Rb-mdm2 interaction.
FAU - Gotz, Claudia
AU  - Gotz C
AD  - Medical Biochemistry and Molecular Biology, University of the Saarland, D-66424 
      Homburg, Germany.
FAU - Kartarius, Sabine
AU  - Kartarius S
FAU - Schwar, Gertrud
AU  - Schwar G
FAU - Montenarh, Mathias
AU  - Montenarh M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 452VLY9402 (Serine)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
RN  - EC 2.7.11.1 (Casein Kinase II)
SB  - IM
MH  - Casein Kinase II/pharmacology
MH  - Humans
MH  - Nuclear Proteins/*metabolism/pharmacology
MH  - Osteosarcoma/*pathology
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins/*metabolism/pharmacology
MH  - Proto-Oncogene Proteins c-mdm2
MH  - Retinoblastoma Protein/*metabolism
MH  - Serine/metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2005/02/11 09:00
MHDA- 2005/06/18 09:00
CRDT- 2005/02/11 09:00
PHST- 2005/02/11 09:00 [pubmed]
PHST- 2005/06/18 09:00 [medline]
PHST- 2005/02/11 09:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2005 Mar;26(3):801-8.