PMID- 15706626
OWN - NLM
STAT- MEDLINE
DCOM- 20050406
LR  - 20131121
IS  - 1076-5174 (Print)
IS  - 1076-5174 (Linking)
VI  - 40
IP  - 2
DP  - 2005 Feb
TI  - Altered extracellular striatal in vivo biotransformation of the opioid 
      neuropeptide dynorphin A(1-17) in the unilateral 6-OHDA rat model of Parkinson's 
      disease.
PG  - 261-70
AB  - The in vivo biotransformation of dynorphin A(1-17) (Dyn A) was studied in the 
      striatum of hemiparkinsonian rats by using microdialysis in combination with 
      nanoflow reversed-phase liquid chromatography/electrospray time-of-flight mass 
      spectrometry. The microdialysis probes were implanted into both hemispheres of 
      unilaterally 6-hydroxydopamine (6-OHDA) lesioned rats. Dyn A (10 pmol microl(-1)) 
      was infused through the probes at 0.4 microl min(-1) for 2 h. Samples were 
      collected every 30 min and analyzed by mass spectrometry. The results showed for 
      the first time that there was a difference in the Dyn A biotransformation when 
      comparing the two corresponding sides of the brain. Dyn A metabolites 1-8, 1-16, 
      5-17, 10-17, 7-10 and 8-10 were detected in the dopamine-depleted striatum but 
      not in the untreated striatum. Dyn A biotransformed fragments found in both 
      hemispheres were N-terminal fragments 1-4, 1-5, 1-6, 1-11, 1-12 and 1-13, 
      C-terminal fragments 2-17, 3-17, 4-17, 7-17 and 8-17 and internal fragments 2-5, 
      2-10, 2-11, 2-12, and 8-15. The relative levels of these fragments were lower in 
      the dopamine-depleted striatum. The results imply that the extracellular in vivo 
      processing of the dynorphin system is being disturbed in the 6-OHDA-lesion animal 
      model of Parkinson's disease.
CI  - Copyright 2005 John Wiley & Sons, Ltd.
FAU - Klintenberg, Rebecka
AU  - Klintenberg R
AD  - Laboratory for Biological and Medical Mass Spectrometry and Department of 
      Pharmaceutical Biosciences, Uppsala University, Box 583, SE-75123 Uppsala, 
      Sweden.
FAU - Andren, Per E
AU  - Andren PE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mass Spectrom
JT  - Journal of mass spectrometry : JMS
JID - 9504818
RN  - 0 (Peptide Fragments)
RN  - 74913-18-1 (Dynorphins)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - N21FAR7B4S (Apomorphine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apomorphine
MH  - Behavior, Animal/drug effects
MH  - Brain Chemistry
MH  - Chromatography, Liquid
MH  - Corpus Striatum/chemistry/*metabolism
MH  - Disease Models, Animal
MH  - Dynorphins/administration & dosage/analysis/*pharmacokinetics
MH  - Mass Spectrometry/methods
MH  - Microdialysis
MH  - Molecular Sequence Data
MH  - Oxidopamine
MH  - Parkinson Disease/*metabolism
MH  - Peptide Fragments/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spectrometry, Mass, Electrospray Ionization
EDAT- 2005/02/12 09:00
MHDA- 2005/04/07 09:00
CRDT- 2005/02/12 09:00
PHST- 2005/02/12 09:00 [pubmed]
PHST- 2005/04/07 09:00 [medline]
PHST- 2005/02/12 09:00 [entrez]
AID - 10.1002/jms.754 [doi]
PST - ppublish
SO  - J Mass Spectrom. 2005 Feb;40(2):261-70. doi: 10.1002/jms.754.