PMID- 15707583
OWN - NLM
STAT- MEDLINE
DCOM- 20050404
LR  - 20181201
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 304
IP  - 1
DP  - 2005 Mar 10
TI  - Regulation of replication at the R/G chromosomal band boundary and 
      pericentromeric heterochromatin of mammalian cells.
PG  - 162-74
AB  - Mammalian chromosomes consist of multiple replicons; however, in contrast to 
      yeast, the details of this replication process (origin firing, fork progression 
      and termination) relative to specific chromosomal domains remain unclear. Using 
      direct visualization of DNA fibers, here we show that the rate of replication 
      fork movement typically decreases in the early-mid S phase when the replication 
      fork proceeds through the R/G chromosomal band boundary and pericentromeric 
      heterochromatin. To support this, fluorescence in situ hybridization (FISH)-based 
      replication profiles at the human 1q31.1 (R-band)-32.1 (G-band) regions revealed 
      that replication timing switched around at the putative R/G chromosomal band 
      boundary predicted by marked changes in GC content at the sequence level. Thus, 
      the slowdown of replication fork movement is thought to be the general property 
      of the band boundaries separating the functionally different chromosomal domains. 
      By simultaneous visualization of replication fork movement and pericentromeric 
      heterochromatin sequences on DNA fibers, we observed that this region is 
      duplicated by many replication forks, some of which proceed unidirectionally, 
      that originate from clustered replication origins. We showed that histone 
      hyperacetylation is tightly associated with changes in the replication timing of 
      pericentromeric heterochromatin induced by 5-aza-2'-deoxycytidine treatment. 
      These results suggest that, similar to the yeast system, histone modification is 
      involved in controlling the timing of origin firing in mammals.
FAU - Takebayashi, Shin-Ichiro
AU  - Takebayashi S
AD  - Laboratory of Molecular and Cellular Biology, Faculty of Bioresources, Mie 
      University, Tsu, Mie 514-8507, Japan. s-take@cdb.riken.jp
FAU - Sugimura, Kazuto
AU  - Sugimura K
FAU - Saito, Tatsuro
AU  - Saito T
FAU - Sato, Chiyoko
AU  - Sato C
FAU - Fukushima, Yoshiyuki
AU  - Fukushima Y
FAU - Taguchi, Hiroshi
AU  - Taguchi H
FAU - Okumura, Katsuzumi
AU  - Okumura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041124
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Heterochromatin)
RN  - 0 (Histones)
RN  - 776B62CQ27 (Decitabine)
RN  - 9007-49-2 (DNA)
RN  - M801H13NRU (Azacitidine)
SB  - IM
MH  - Animals
MH  - Azacitidine/*analogs & derivatives/pharmacology
MH  - Cell Line
MH  - Centromere/*physiology
MH  - Chromosome Banding
MH  - DNA/biosynthesis
MH  - *DNA Replication
MH  - Decitabine
MH  - Female
MH  - HeLa Cells
MH  - Heterochromatin/*physiology
MH  - Histones/metabolism
MH  - Humans
MH  - Interphase
MH  - Mice
MH  - Mitosis
MH  - S Phase
EDAT- 2005/02/15 09:00
MHDA- 2005/04/05 09:00
CRDT- 2005/02/15 09:00
PHST- 2004/05/23 00:00 [received]
PHST- 2004/10/17 00:00 [revised]
PHST- 2004/10/27 00:00 [accepted]
PHST- 2005/02/15 09:00 [pubmed]
PHST- 2005/04/05 09:00 [medline]
PHST- 2005/02/15 09:00 [entrez]
AID - S0014-4827(04)00655-X [pii]
AID - 10.1016/j.yexcr.2004.10.024 [doi]
PST - ppublish
SO  - Exp Cell Res. 2005 Mar 10;304(1):162-74. doi: 10.1016/j.yexcr.2004.10.024. Epub 
      2004 Nov 24.