PMID- 15709025
OWN - NLM
STAT- MEDLINE
DCOM- 20050331
LR  - 20220424
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 79
IP  - 5
DP  - 2005 Mar
TI  - Processing and presentation of exogenous HLA class I peptides by dendritic cells 
      from human immunodeficiency virus type 1-infected persons.
PG  - 3052-62
AB  - Dendritic cells (DCs) loaded with viral peptides are a potential form of 
      immunotherapy of human immunodeficiency virus type 1 (HIV-1) infection. We show 
      that DCs derived from blood monocytes of subjects with chronic HIV-1 infection on 
      combination antiretroviral drug therapy have increases in expression of HLA, 
      T-cell coreceptor, and T-cell activation molecules in response to the DC 
      maturation factor CD40L comparable to those from uninfected persons. Mature DCs 
      (mDCs) loaded with HLA A*0201-restricted viral peptides of the optimal length 
      (9-mer) were more efficient at activating antiviral CD8(+) T cells than were 
      immature DCs or peptide alone. Optimal presentation of these exogenous peptides 
      required uptake and vesicular trafficking and was comparable in DCs derived from 
      HIV-1-infected and uninfected persons. Furthermore, DCs from HIV-1-infected and 
      uninfected persons had similar capacities to process viral peptides with 
      C-terminal and N-terminal extensions through their proteasomal and cytosolic 
      pathways, respectively. We conclude that DCs derived from HIV-1-infected persons 
      have similar abilities to process exogenous peptides for presentation to CD8(+) T 
      cells as those from uninfected persons. This conclusion supports the use of DCs 
      loaded with synthetic peptides in immunotherapy of HIV-1 infection.
FAU - Huang, Xiao-Li
AU  - Huang XL
AD  - University of Pittsburgh Graduate School of Public Health, 130 DeSoto St., 
      Pittsburgh, PA 15261, USA.
FAU - Fan, Zheng
AU  - Fan Z
FAU - Colleton, Bonnie A
AU  - Colleton BA
FAU - Buchli, Rico
AU  - Buchli R
FAU - Li, Hongyi
AU  - Li H
FAU - Hildebrand, William H
AU  - Hildebrand WH
FAU - Rinaldo, Charles R Jr
AU  - Rinaldo CR Jr
LA  - eng
GR  - U01 AI037984/AI/NIAID NIH HHS/United States
GR  - R01 AI041870/AI/NIAID NIH HHS/United States
GR  - U01 AI37984/AI/NIAID NIH HHS/United States
GR  - R01 AI41870/AI/NIAID NIH HHS/United States
GR  - U01 AI035041/AI/NIAID NIH HHS/United States
GR  - U01 AI35041/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antimetabolites)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Oligopeptides)
SB  - IM
MH  - Antigen Presentation/drug effects
MH  - Antimetabolites/pharmacology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Case-Control Studies
MH  - Dendritic Cells/*immunology
MH  - HIV Infections/*immunology
MH  - HIV-1
MH  - HLA Antigens/*metabolism
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Oligopeptides/immunology
PMC - PMC548465
EDAT- 2005/02/15 09:00
MHDA- 2005/04/01 09:00
PMCR- 2005/03/01
CRDT- 2005/02/15 09:00
PHST- 2005/02/15 09:00 [pubmed]
PHST- 2005/04/01 09:00 [medline]
PHST- 2005/02/15 09:00 [entrez]
PHST- 2005/03/01 00:00 [pmc-release]
AID - 79/5/3052 [pii]
AID - 1908-04 [pii]
AID - 10.1128/JVI.79.5.3052-3062.2005 [doi]
PST - ppublish
SO  - J Virol. 2005 Mar;79(5):3052-62. doi: 10.1128/JVI.79.5.3052-3062.2005.