PMID- 15709423
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20231213
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 22
IP  - 4
DP  - 2004 Jul
TI  - [Simultaneous determination of neopterin and biopterin in urine by high 
      performance liquid chromatography].
PG  - 416-9
AB  - A method was established for simultaneous determination of neopterin (NP) and 
      biopterin (BP) in urine by high performance liquid chromatography-fluorescence 
      detection. CONDITIONS: column, Hypersil BDS C18 (4.6 mm i. d. x 200 mm, 5 
      microm); mobile phase, methanol-water (10:90, v/v); flow rate, 0.5 mL/min; column 
      temperature, 20 degrees C; excitation wavelength, 360 nm; emission wavelength, 
      440 nm. Urine samples were first precipitated with trichloroacetic acid, then 
      centrifuged at 12 000 r/min for 15 min at 4 degrees C, and finally added with 
      NaOH to neutralize the supernatant. Injection volume was 30 microL. The linear 
      ranges of determination of the standards NP and BP were 0.12 - 1000 microg/L and 
      0.48 -1000 microg/L, with the detection limits of 0.01 microg/L and 0.1 mictog/L, 
      the relative standard deviations (RSDs) in urine of 1.09% and 3.39%, and average 
      recoveries of 100.7% and 100.3%, respectively. The concentrations of NP and BP, 
      and concentration ratio of NP and BP in urines were detected and compared between 
      controls and uremia patients. The concentration of creatinine (Cr) in urines was 
      detected with picric acid method. The concentration ratios of NP and Cr, NP and 
      BP in uremia group increased more significantly than that in normal group (P < 
      0.05). The method is simple, fast, accurate and convenient. It can be used to 
      detect NP and BP for clinical urine samples and provide a more convenient and 
      accurate tool for the diagnosis of patients.
FAU - Zhang, Mingjun
AU  - Zhang M
AD  - Department of Medical Laboratory, Chongqing University of Medical Sciences, 
      Chongqing 400016, China. 23abc_zhang@163.com
FAU - Ding, Shijia
AU  - Ding S
FAU - Yi, Gang
AU  - Yi G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Biopterins)
RN  - 670-65-5 (Neopterin)
SB  - IM
MH  - Biopterins/*urine
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Neopterin/*urine
MH  - Spectrometry, Fluorescence
EDAT- 2005/02/16 09:00
MHDA- 2007/06/20 09:00
CRDT- 2005/02/16 09:00
PHST- 2005/02/16 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2005/02/16 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2004 Jul;22(4):416-9.