PMID- 15709425
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20181201
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 22
IP  - 4
DP  - 2004 Jul
TI  - [Analysis for four isomers of resveratrol in red wine by high performance liquid 
      chromatography].
PG  - 424-7
AB  - Red wine was treated by hydrolysis with beta-glucosidase, so the piceids in it 
      were converted to resveratrols. The contents of cis- and trans-resveratrols 
      before and after hydrolysis were determined by high performance liquid 
      chromatography, thereby the contents of the four isomers of resveratrol in the 
      red wine can be calculated. The analysis was performed on a Symmetry C18 column 
      (3.9 mm i. d. x 150 mm) with a mixture of ethanol and 0.05% acetic acid solution 
      (23:77, v/v) as the mobile phase at a flow rate of 1.0 mL/min and with UV 
      detection at 306 nm. Results show that there was a good linear relationship (r > 
      0.999) for all the four isomers of resveratrol in the range of 0.2 - 50.0 mg/L. 
      The detection limits of cis- and trans-resveratrol and the corresponding piceid 
      isomers were 0.81, 1.20, 0.40 and 0.46 ng, respectively. The recovery of 
      resveratrol was more than 98%. Relative standard deviations (RSDs) (n = 5) were 
      from 0.5% to 8.5%. The ranges of concentration of total resveratrol were 0.84 - 
      7.33 mg/L in eleven dry red wines and 0.12 - 6.00 mg/L in three grape juices. The 
      method is convenient, and there are good precision and repeatability. The method 
      is applicable for the analysis of four isomers of resveratrol in red wines and 
      grape juices.
FAU - Di, Sun
AU  - Di S
AD  - Beijing Deltagen Biological Technology Co., Ltd., Beijing 100085, China. 
      sundi-deltagen@163.net
FAU - Zhang, Zhenxing
AU  - Zhang Z
FAU - Wang, Yinan
AU  - Wang Y
FAU - Shi, Weiping
AU  - Shi W
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Stilbenes)
RN  - EC 3.2.1.21 (beta-Glucosidase)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Resveratrol
MH  - Stereoisomerism
MH  - Stilbenes/analysis/*chemistry
MH  - Wine/*analysis
MH  - beta-Glucosidase
EDAT- 2005/02/16 09:00
MHDA- 2007/06/20 09:00
CRDT- 2005/02/16 09:00
PHST- 2005/02/16 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2005/02/16 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2004 Jul;22(4):424-7.