PMID- 1571090
OWN - NLM
STAT- MEDLINE
DCOM- 19920601
LR  - 20180215
IS  - 1015-2008 (Print)
IS  - 1015-2008 (Linking)
VI  - 60
IP  - 2
DP  - 1992
TI  - Interleukin-4 differentially regulates tumor necrosis factor-alpha gene 
      expression by human T lymphocytes and monocytes.
PG  - 100-7
AB  - Tumor necrosis factor-alpha (TNF-alpha), a product of both mononuclear phagocytes 
      and T lymphocytes, is an important proximal mediator of a number of acute and 
      chronic inflammatory disease states. In this investigation we examine the 
      regulatory effects of the lymphocyte product interleukin-4 (IL-4) on the gene 
      expression of TNF-alpha from stimulated human peripheral blood monocytes (PBM) 
      and T lymphocytes. We demonstrated the dose-dependent suppression of TNF-alpha 
      mRNA and protein synthesis from lipopolysaccharide-treated PBM by IL-4. The 
      suppressive effects of IL-4 appear to be dependent upon de novo protein 
      synthesis, as cycloheximide abrogated the IL-4-induced reduction in TNF-alpha 
      mRNA levels from PBM. In contrast to the suppressive effects of IL-4 on 
      PBM-derived cytokine expression, IL-4 did not alter TNF-alpha mRNA expression 
      from alpha-Cd3 or PMA + alpha-CD-28-treated T lymphocytes. Moreover, IL-2 mRNA 
      expression from similarly treated T lymphocytes was unaltered by IL-4. Our 
      findings demonstrate that disparity exists in the regulation of TNF-alpha gene 
      expression from different immune cell populations which may have important 
      implications in the evolution of acute and chronic inflammatory responses.
FAU - Standiford, T J
AU  - Standiford TJ
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor 
      48109-0602.
FAU - Lindsten, T
AU  - Lindsten T
FAU - Thompson, C B
AU  - Thompson CB
FAU - Strieter, R M
AU  - Strieter RM
FAU - Kunkel, S L
AU  - Kunkel SL
LA  - eng
GR  - HL102401/HL/NHLBI NIH HHS/United States
GR  - HL31693/HL/NHLBI NIH HHS/United States
GR  - HL35276/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
RN  - 0 (Endotoxins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Base Sequence
MH  - Cycloheximide/pharmacology
MH  - Depression, Chemical
MH  - Endotoxins/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Interleukin-4/*pharmacology
MH  - Leukocytes, Mononuclear/*drug effects
MH  - Lymphocyte Activation
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis
MH  - T-Lymphocytes/*drug effects
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/genetics
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1159/000163706 [doi]
PST - ppublish
SO  - Pathobiology. 1992;60(2):100-7. doi: 10.1159/000163706.