PMID- 15711982
OWN - NLM
STAT- MEDLINE
DCOM- 20051031
LR  - 20171116
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 17
IP  - 12
DP  - 2005 May
TI  - The active role played by xenogeneic endothelial cells in the indirect 
      presentation pathway is not lymphocyte trans-co-stimulation.
PG  - 787-94
AB  - The human CD4+ T lymphocyte response to major histocompatibility complex (MHC) 
      class II-negative porcine endothelial cells is dependent on the presence of human 
      monocytes through a human leukocyte antigen (HLA) class II-restricted indirect 
      presentation pathway. Because the role of porcine endothelial cells had been 
      previously shown to do more than simply supply xenopeptides, co-stimulatory 
      signals were analysed. Endothelial cells were shown to express the CD54, CD58, 
      CD59 and CD86 transcripts; however, no membrane B7 molecule could be detected. 
      Blocking experiments in a direct pathway model confirmed that porcine endothelial 
      cells could provide co-stimulatory signals to human T cells through the CD2 and 
      LFA-1 pathways. Nevertheless, the proliferation achieved in the indirect 
      presentation model required co-stimulation by LFA-1, CD2 and CD28, engaged by 
      co-stimulation molecules expressed in the cis-form by the human monocytes. These 
      results clearly show that the active role played by the endothelial cells in the 
      indirect pathway is not lymphocyte trans-co-stimulation and suggest that 
      cis-co-stimulation dominates trans-co-stimulation when both are present.
FAU - Brossay, Angelique
AU  - Brossay A
AD  - EA 3249 Cellules Hematopoietiques, Hemostase et Greffe and IFR 135 Imagerie et 
      Exploration fonctionelles, Universite Francois Rabelais, Tours, France.
FAU - Harang, Solene
AU  - Harang S
FAU - Herault, Olivier
AU  - Herault O
FAU - Bardos, Pierre
AU  - Bardos P
FAU - Watier, Herve
AU  - Watier H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050215
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Heterophile)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD2 Antigens)
RN  - 0 (CD48 Antigen)
RN  - 0 (CD58 Antigens)
RN  - 0 (CD59 Antigens)
RN  - 0 (CD86 protein, human)
RN  - 0 (Immunoconjugates)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 7D0YB67S97 (Abatacept)
SB  - IM
MH  - Abatacept
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD/genetics/immunology
MH  - Antigens, Heterophile/*immunology
MH  - Aorta/cytology
MH  - B7-1 Antigen/genetics/immunology
MH  - B7-2 Antigen
MH  - CD2 Antigens/immunology/metabolism
MH  - CD48 Antigen
MH  - CD58 Antigens/genetics/immunology
MH  - CD59 Antigens/genetics/immunology
MH  - Cells, Cultured
MH  - Endothelial Cells/cytology/*immunology
MH  - Humans
MH  - Immunoconjugates/immunology/metabolism
MH  - Intercellular Adhesion Molecule-1/genetics/immunology
MH  - Lymphocytes/cytology/*immunology
MH  - Membrane Glycoproteins/genetics/immunology
MH  - RNA, Messenger/analysis
MH  - Swine
MH  - Swine, Miniature
MH  - Transplantation, Heterologous/*immunology
EDAT- 2005/02/16 09:00
MHDA- 2005/11/01 09:00
CRDT- 2005/02/16 09:00
PHST- 2003/03/24 00:00 [received]
PHST- 2003/09/04 00:00 [accepted]
PHST- 2003/08/29 00:00 [revised]
PHST- 2005/02/16 09:00 [pubmed]
PHST- 2005/11/01 09:00 [medline]
PHST- 2005/02/16 09:00 [entrez]
AID - 10.1007/s00147-004-0773-9 [doi]
PST - ppublish
SO  - Transpl Int. 2005 May;17(12):787-94. doi: 10.1007/s00147-004-0773-9. Epub 2005 
      Feb 15.