PMID- 15712252
OWN - NLM
STAT- MEDLINE
DCOM- 20050916
LR  - 20171116
IS  - 1099-498X (Print)
IS  - 1099-498X (Linking)
VI  - 7
IP  - 7
DP  - 2005 Jul
TI  - Cytokine production by dendritic cells genetically engineered to express IL-4: 
      induction of Th2 responses and differential regulation of IL-12 and IL-23 
      synthesis.
PG  - 869-77
AB  - Dendritic cells (DCs) retrovirally transduced with IL-4 have recently been shown 
      to inhibit murine collagen-induced arthritis and associated Th1 immune responses 
      in vivo, but the mechanisms that underly these effects are not yet understood. In 
      this report we demonstrate that IL-4-transduced DCs loaded with antigen led to 
      lower T cell production of IFN-gamma, increased production of IL-4, and an 
      attenuated, delayed type hypersensitivity response. We hypothesized that the 
      ability of such DCs to regulate the Th1 immune response in vivo depends in part 
      on their capacity to produce IL-12 and IL-23. Quantitative mRNA analysis revealed 
      that IL-4-transduced DCs stimulated with CD40 ligand expressed higher levels of 
      IL-12p35 mRNA, but lower levels of mRNA for IL-23p19 and the common subunit p40 
      found in both IL-12 and IL-23, compared with control DCs. These results, which 
      indicate that expression of the IL-12 and IL-23 subunits is differentially 
      regulated in IL-4-transduced DCs, were confirmed by ELISA of the IL-12 and IL-23 
      heterodimers. Thus, therapeutic suppression of Th1 -mediated autoimmunity (as 
      recently shown in murine collagen-induced arthritis) and induction of Th2 
      responses in vivo by IL-4-transduced DCs occurs despite their potential to 
      produce increased levels of IL-12, but could reflect, in part, decreased 
      production of IL-23.
CI  - Copyright 2005 John Wiley & Sons, Ltd.
FAU - Morita, Yoshitaka
AU  - Morita Y
AD  - Division of Nephrology and Rheumatology, Department of Internal Medicine, 
      Kawasaki Medical School, Kurashiki, Japan.
FAU - Gupta, Raj
AU  - Gupta R
FAU - Seidl, Kelly M
AU  - Seidl KM
FAU - McDonagh, Kevin T
AU  - McDonagh KT
FAU - Fox, David A
AU  - Fox DA
LA  - eng
GR  - AR38477/AR/NIAMS NIH HHS/United States
GR  - DK02349/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Gene Med
JT  - The journal of gene medicine
JID - 9815764
RN  - 0 (Cytokines)
RN  - 0 (Il23a protein, mouse)
RN  - 0 (Interleukin-23)
RN  - 0 (Interleukin-23 Subunit p19)
RN  - 0 (Interleukins)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 9013-72-3 (Hemocyanins)
RN  - FV4Y0JO2CX (keyhole-limpet hemocyanin)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - CD40 Ligand/genetics
MH  - Cytokines/biosynthesis/*immunology
MH  - Dendritic Cells/*immunology/metabolism
MH  - *Gene Transfer Techniques
MH  - Hemocyanins/immunology
MH  - Interleukin-12/*biosynthesis/metabolism
MH  - Interleukin-23
MH  - Interleukin-23 Subunit p19
MH  - Interleukin-4/*genetics/immunology
MH  - Interleukins/*biosynthesis/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NIH 3T3 Cells
MH  - Th2 Cells/*immunology/metabolism
MH  - Transfection
EDAT- 2005/02/16 09:00
MHDA- 2005/09/17 09:00
CRDT- 2005/02/16 09:00
PHST- 2005/02/16 09:00 [pubmed]
PHST- 2005/09/17 09:00 [medline]
PHST- 2005/02/16 09:00 [entrez]
AID - 10.1002/jgm.730 [doi]
PST - ppublish
SO  - J Gene Med. 2005 Jul;7(7):869-77. doi: 10.1002/jgm.730.