PMID- 15712289
OWN - NLM
STAT- MEDLINE
DCOM- 20050512
LR  - 20191210
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 19
IP  - 6
DP  - 2005
TI  - Determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 
      3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxymethamphetamine in 
      urine by online solid-phase extraction and ion-pairing liquid chromatography with 
      detection by electrospray tandem mass spectrometry.
PG  - 775-80
AB  - A method using an online solid-phase extraction (SPE) and ion-pairing liquid 
      chromatography with electrospray tandem mass spectrometry (LC/ES-MS/MS) was 
      developed for determination of amphetamine (Amp), methamphetamine (mAmp), 
      3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxyethylamphetamine (MDEA), 
      and 3,4-methylenedioxymethamphetamine (MDMA) in urine samples. A SPE cartridge 
      column with both hydrophilic and lipophilic functions was utilized for online 
      extraction. A reversed-phase C18 LC column was employed for LC separation and 
      MS/MS was used for detection. Trifluoroacetic acid was added to the mobile phase 
      as an ion-pairing reagent. This method was fully automated and the extraction and 
      analysis procedures were controlled by a six-port switch valve. Recoveries 
      ranging from 85-101% were measured. Good linear ranges (10-500 ng/mL) for Amp and 
      mAmp were determined. For MDA, MDMA and MDEA, dual linear ranges were obtained 
      from 5-100 and 100-500 ng/mL, respectively. The detection limit of each 
      analytical compound, based on a signal-to-noise ratio of 3, ranged from 1-3 
      ng/mL. The applicability of this newly developed method was examined by analyzing 
      several urine samples from drug users. Good agreement was obtained between the 
      results from this method and a literature GC/MS method.
FAU - Wu, Ti-Yu
AU  - Wu TY
AD  - P.O. Box 86-72, Department of Chemistry, Soochow University, Taipei, Taiwan.
FAU - Fuh, Ming-Ren
AU  - Fuh MR
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Amphetamines)
SB  - IM
MH  - Amphetamines/*urine
MH  - Chemical Fractionation/methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Microchemistry/methods
MH  - Online Systems
MH  - Reproducibility of Results
MH  - Robotics/methods
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Substance Abuse Detection/*methods
MH  - Substance-Related Disorders/*diagnosis/*urine
MH  - Urinalysis/*methods
EDAT- 2005/02/16 09:00
MHDA- 2005/05/13 09:00
CRDT- 2005/02/16 09:00
PHST- 2005/02/16 09:00 [pubmed]
PHST- 2005/05/13 09:00 [medline]
PHST- 2005/02/16 09:00 [entrez]
AID - 10.1002/rcm.1856 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2005;19(6):775-80. doi: 10.1002/rcm.1856.