PMID- 15712897
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 22
IP  - 3
DP  - 2004 May
TI  - [Determination of antisense phosphorothioate oligonucleotide drug Cantide by 
      capillary gel electrophoresis].
PG  - 202-5
AB  - Cantide is a 20-mer antisense phosphorothioate oligonucleotide that inhibits 
      telomerase catalytic subunit hTERT. A capillary gel electrophoresis (CGE) method 
      with internal standard was used for the determination of Cantide. Cantide and the 
      phosphorothioate internal standard were prepared on a solid-phase DNA synthesizer 
      and purified by preparative strong anion-exchange chromatography and 
      reversed-phase high performance liquid chromatography. Cantide and the internal 
      standard had approximately equal percentage of base composition. Cantide was 
      determined with capillary electrophoresis instrument and ssDNA kit. The size of 
      the capillary column was 31 cm x 100 microm i.d. with an effective length of 20 
      cm. Samples were electrokinetically injected using -10 kV voltage for a duration 
      of 1 s. The column temperature and sample storage temperature was 40 degrees C 
      and 30 degrees C, respectively. The detection wavelength was 254 nm. The running 
      buffer was a mixture of 7 mol/L urea-tris-boric acid (pH 8.5). The calibration 
      curve was linear in the range of 12.5-800 mg/L with correlation coefficient of 
      0.99997. The limit of detection of Cantide was 0.220 mg/L. Intra-day and 
      inter-day relative standard deviations (RSDs) for Cantide were 0.449%-1.89%, and 
      1.01%-1.48%, respectively. The average recovery was 96.95% with RSD of 6.88%. The 
      assay validation study and the characterization of CGE revealed that the method 
      can be used in quantitative analysis of Cantide for pharmacokinetic 
      characterization, and the results are accurate and reproducible.
FAU - Yang, Binghu
AU  - Yang B
AD  - Beijing Institute of Radiation Medicine, Beijing 100850, China. 
      yangbinghu@sina.com
FAU - Sun, Oujun
AU  - Sun O
FAU - Zhang, Minli
AU  - Zhang M
FAU - Wang, Shengqi
AU  - Wang S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Phosphorothioate Oligonucleotides)
RN  - 0 (cantide)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - *Electrophoresis, Agar Gel/methods
MH  - *Electrophoresis, Capillary/methods
MH  - Phosphorothioate Oligonucleotides/*analysis/*chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Extraction
MH  - Telomerase/antagonists & inhibitors
EDAT- 2005/02/17 09:00
MHDA- 2010/06/04 06:00
CRDT- 2005/02/17 09:00
PHST- 2005/02/17 09:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
PHST- 2005/02/17 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2004 May;22(3):202-5.