PMID- 15712904
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 22
IP  - 3
DP  - 2004 May
TI  - [Determination of L-cysteine in enzymatic reaction mixture with pre-column 
      derivatization by high performance liquid chromatography].
PG  - 231-3
AB  - A new sensitive high performance liquid chromatographic method for the 
      determination of L-cysteine in an enzymatic reaction mixture using ultra violet 
      spectrometric detection was developed. The sample reacted with 
      5,5'-dithio-bis-nitrobenzoic acid (DTNB) and was analyzed on a Shimadzu VP-ODS 
      column at room temperature, using gradient elution with detection at 330 nm. The 
      L-cysteine chromatographic peak was determined in comparison with derivatives of 
      2-mercapto ethanol and dithiothreitol. The linear range was 5-950 micromol/L. The 
      recoveries were 99.7%-100.5% and the relative standard deviations (RSDs) were 
      less than 1.3%. The detection limit was 0.8 micromol/L. The method is simple and 
      accurate.
FAU - Liu, Zhong
AU  - Liu Z
AD  - College of Life Science, Nankai University, Tianjin 300071, China. 
      liuzhong@eyou.com
FAU - Yang, Wenbo
AU  - Yang W
FAU - Jin, Yongjie
AU  - Jin Y
FAU - Bai, Gang
AU  - Bai G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 60-24-2 (Mercaptoethanol)
RN  - 9BZQ3U62JX (Dithionitrobenzoic Acid)
RN  - K848JZ4886 (Cysteine)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - *Chromatography, High Pressure Liquid/methods
MH  - Cysteine/*analysis/chemistry
MH  - Dithionitrobenzoic Acid/analysis
MH  - Dithiothreitol/analysis
MH  - Mercaptoethanol/analysis
MH  - Photoelectron Spectroscopy/methods
EDAT- 2005/02/17 09:00
MHDA- 2010/06/04 06:00
CRDT- 2005/02/17 09:00
PHST- 2005/02/17 09:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
PHST- 2005/02/17 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2004 May;22(3):231-3.