PMID- 15712910
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 22
IP  - 3
DP  - 2004 May
TI  - [Determination of aceclofenac in human plasma by reversed-phase high performance 
      liquid chromatography].
PG  - 252-4
AB  - A simple and rapid reversed-phase high performance liquid chromatographic 
      (RP-HPLC) method for the determination of aceclofenac in human plasma has been 
      established. Plasma samples from 18 male volunteers were collected. The samples 
      were extracted with ether, and the extractant was redissolved in the mobile phase 
      after dried by N2 at 37 degrees C. Chromatography was performed on an ODS column 
      with methanol-0.1 mol/L ammonium acetate (pH 6.0) (7:3, v/v) as the mobile phase. 
      The flow rate was 1.0 mL/min. The UV-Vis detector was set at 275 nm. The linear 
      range was from 0.05 mg/L to 40.0 mg/L with a correlation coefficient of 0.9999. 
      The intra-day and inter-day relative standard deviations (RSDs) for assaying the 
      plasma samples containing three levels of aceclofenac were 1.84%-7.13% and 
      3.40%-9.33% (n = 5), respectively. The mean absolute recovery was 82.5% and the 
      mean relative recovery was 100.3%. This sensitive and selective method can be 
      used for clinical pharmacokinetic study of aceclofenac.
FAU - Jin, You
AU  - Jin Y
AD  - Department of Pharmacology, Tongji Medical College of Huazhong University of 
      Science and Technology, Wuhan 430030, China. jinyou81@hotmail.com
FAU - Chen, Hui
AU  - Chen H
FAU - Gu, Shifen
AU  - Gu S
FAU - Zeng, Fandian
AU  - Zeng F
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Indicators and Reagents)
RN  - 144O8QL0L1 (Diclofenac)
RN  - RPK779R03H (aceclofenac)
SB  - IM
MH  - Adult
MH  - Anti-Inflammatory Agents, Non-Steroidal/*blood
MH  - *Chromatography, High Pressure Liquid/methods
MH  - *Chromatography, Reverse-Phase/methods
MH  - Diclofenac/*analogs & derivatives/blood
MH  - Humans
MH  - Indicators and Reagents
MH  - Male
EDAT- 2005/02/17 09:00
MHDA- 2010/06/04 06:00
CRDT- 2005/02/17 09:00
PHST- 2005/02/17 09:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
PHST- 2005/02/17 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2004 May;22(3):252-4.