PMID- 15713617 OWN - NLM STAT- MEDLINE DCOM- 20050310 LR - 20200613 IS - 0022-2615 (Print) IS - 0022-2615 (Linking) VI - 54 IP - Pt 3 DP - 2005 Mar TI - Influence of antimicrobial treatment for Helicobacter pylori infection on the intestinal microflora in Japanese macaques. PG - 309-314 LID - 10.1099/jmm.0.45814-0 [doi] AB - Eradication treatment for Helicobacter pylori is known to cause mild but relatively frequent adverse effects. Some adverse effects such as diarrhoea and soft stools are related to disruption of the composition of the intestinal microflora. This study investigated the microfloral changes resulting from administration of an eradication regimen using proton pump inhibitor (PPI), amoxycillin and clarithromycin. Twenty-eight laboratory-bred Japanese macaques either were administered eradication treatment by this regimen for 7 days or received no medication. Faecal samples were collected for analysis on days 0, 8 and 15, and both aerobic and anaerobic cultures were performed. Among aerobic bacteria, Streptococcus had significantly decreased by day 8, while Enterococcus and Enterobacteriaceae had significantly increased. However, the total number of aerobic bacteria was not significantly decreased from pretreatment levels 1 day after completion of treatment. The number of anaerobic bacteria did not change significantly by day 8. However, the number of Lactobacillus and the detection rate of Bifidobacterium, Peptostreptococcus and Veillonella significantly decreased by day 8, although the number of Bifidobacterium, Peptostreptococcus and Veillonella had almost recovered up to the pretreatment levels 1 week after completion of treatment (day 15). These results suggest that the alterations in the composition of the intestinal microflora caused by the antimicrobial regimen that excludes metronidazole are different from those caused by the regimen including this drug. However, the alterations in bacterial microflora had almost reversed 7 days after completion of treatment in these macaques, which supports clinical findings that diarrhoea or soft stools in humans resolve relatively quickly after a similar treatment. FAU - Tanaka, Junji AU - Tanaka J AD - Division of Gastroenterology, Department of Internal Medicine, Hyogo Medical College, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan 2Division of Gastroenterology, Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan. FAU - Fukuda, Yoshihiro AU - Fukuda Y AD - Division of Gastroenterology, Department of Internal Medicine, Hyogo Medical College, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan 2Division of Gastroenterology, Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan. FAU - Shintani, Shigeyuki AU - Shintani S AD - Division of Gastroenterology, Department of Internal Medicine, Hyogo Medical College, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan 2Division of Gastroenterology, Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan. FAU - Hori, Kazutoshi AU - Hori K AD - Division of Gastroenterology, Department of Internal Medicine, Hyogo Medical College, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan 2Division of Gastroenterology, Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan. FAU - Tomita, Toshihiko AU - Tomita T AD - Division of Gastroenterology, Department of Internal Medicine, Hyogo Medical College, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan 2Division of Gastroenterology, Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan. FAU - Ohkusa, Toshifumi AU - Ohkusa T AD - Division of Gastroenterology, Department of Internal Medicine, Hyogo Medical College, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan 2Division of Gastroenterology, Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan. FAU - Matsumoto, Takayuki AU - Matsumoto T AD - Division of Gastroenterology, Department of Internal Medicine, Hyogo Medical College, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan 2Division of Gastroenterology, Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan. FAU - Miwa, Hiroto AU - Miwa H AD - Division of Gastroenterology, Department of Internal Medicine, Hyogo Medical College, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501, Japan 2Division of Gastroenterology, Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan. LA - eng PT - Journal Article PL - England TA - J Med Microbiol JT - Journal of medical microbiology JID - 0224131 RN - 0 (2-Pyridinylmethylsulfinylbenzimidazoles) RN - 0 (Anti-Infective Agents) RN - 0 (Proton Pump Inhibitors) RN - 0K5C5T2QPG (Lansoprazole) RN - 804826J2HU (Amoxicillin) RN - H1250JIK0A (Clarithromycin) RN - KG60484QX9 (Omeprazole) SB - IM MH - 2-Pyridinylmethylsulfinylbenzimidazoles MH - Amoxicillin/pharmacology/therapeutic use MH - Animals MH - Anti-Infective Agents/pharmacology/*therapeutic use MH - Bacteria, Aerobic/drug effects/growth & development MH - Bacteria, Anaerobic/drug effects/growth & development MH - Clarithromycin/pharmacology/therapeutic use MH - Enterobacteriaceae/drug effects/growth & development MH - Enterococcus/drug effects/growth & development MH - Feces/microbiology MH - Helicobacter Infections/*drug therapy/microbiology MH - Helicobacter pylori/*drug effects/growth & development MH - Intestines/*microbiology MH - Lansoprazole MH - Macaca MH - Omeprazole/*analogs & derivatives/pharmacology/therapeutic use MH - Proton Pump Inhibitors EDAT- 2005/02/17 09:00 MHDA- 2005/03/11 09:00 CRDT- 2005/02/17 09:00 PHST- 2005/02/17 09:00 [pubmed] PHST- 2005/03/11 09:00 [medline] PHST- 2005/02/17 09:00 [entrez] AID - 10.1099/jmm.0.45814-0 [doi] PST - ppublish SO - J Med Microbiol. 2005 Mar;54(Pt 3):309-314. doi: 10.1099/jmm.0.45814-0.