PMID- 15714060
OWN - NLM
STAT- MEDLINE
DCOM- 20050630
LR  - 20191109
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 14
IP  - 1
DP  - 2005 Mar
TI  - Molecular diagnosis of Ewing's sarcoma/primitive neuroectodermal tumor in 
      formalin-fixed paraffin-embedded tissues by RT-PCR and fluorescence in situ 
      hybridization.
PG  - 23-8
AB  - Recent studies have suggested that formalin-fixed paraffin-embedded (FFPE) 
      tissues can be used for molecular analyses by fluorescence in situ hybridization 
      (FISH) and RT-PCR. We analyzed 18 cases of ES/PNET for the t(11;22)(q24;q12) and 
      t(21;22)(q22;q12) fusion transcripts by RT-PCR and analyzed for EWS translocation 
      by interphase FISH with a dual color fusion probe to compare these two approaches 
      directly. RT-PCR detected 13 (72%) EWS-FLI-1 fusions (type I=10, type II=3) and 2 
      (11%) EWS-ERG fusions. Three cases could not be evaluated because the 
      housekeeping gene phosphoglycerate kinase (internal mRNA control) was not 
      amplified. FISH was diagnostic in 15 of 18 cases (83%). There were three 
      discordant cases between RT-PCR and FISH (concordance of 83%). Using a 
      combination of RT-PCR and FISH, the results were complementary. One advantage of 
      RT-PCR analysis was that subtypes of EWS translocation could be determined 
      specifically (type I, type II and ERG). These findings indicate that because of 
      the difficulties and limitations associated with the molecular analysis of FFPE 
      tissues, a combination of RT-PCR and FISH may be a better approach to enhance the 
      sensitivity and accuracy of detecting ES/PNET translocations in FFPE tissues with 
      suboptimally preserved nucleic acids.
FAU - Qian, Xiang
AU  - Qian X
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, 
      Minnesota 55905, USA.
FAU - Jin, Long
AU  - Jin L
FAU - Shearer, Brandon M
AU  - Shearer BM
FAU - Ketterling, Rhett P
AU  - Ketterling RP
FAU - Jalal, Syed M
AU  - Jalal SM
FAU - Lloyd, Ricardo V
AU  - Lloyd RV
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (EWS-ERG fusion protein, human)
RN  - 0 (EWS-FLI fusion protein)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Transcription Factors)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 21/genetics
MH  - Chromosomes, Human, Pair 22/genetics
MH  - DNA Primers/genetics
MH  - DNA, Neoplasm/genetics
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods/statistics & numerical data
MH  - Interphase
MH  - Neuroectodermal Tumors, Primitive, Peripheral/*diagnosis/*genetics
MH  - Oncogene Proteins, Fusion/genetics
MH  - Paraffin Embedding
MH  - Proto-Oncogene Protein c-fli-1
MH  - RNA-Binding Protein EWS
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods/statistics & numerical 
      data
MH  - Sarcoma, Ewing/*diagnosis/*genetics
MH  - Sensitivity and Specificity
MH  - Tissue Fixation
MH  - Transcription Factors/genetics
MH  - Translocation, Genetic
EDAT- 2005/02/17 09:00
MHDA- 2005/07/01 09:00
CRDT- 2005/02/17 09:00
PHST- 2005/02/17 09:00 [pubmed]
PHST- 2005/07/01 09:00 [medline]
PHST- 2005/02/17 09:00 [entrez]
AID - 00019606-200503000-00004 [pii]
AID - 10.1097/01.pdm.0000140192.27878.97 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2005 Mar;14(1):23-8. doi: 10.1097/01.pdm.0000140192.27878.97.