PMID- 15714819
OWN - NLM
STAT- MEDLINE
DCOM- 20050719
LR  - 20221207
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 30
IP  - 2
DP  - 2005 Jan
TI  - [Pharmacokinetics of oxymatrine and its metabolite in beagle dogs by LC-MS].
PG  - 133-6
AB  - OBJECTIVE: To establish LC-MS method in the determination of oxymatrine and its 
      metabolite in plasma and investigate their pharmacokinetics in beagle dogs. 
      METHOD: Lichrospher C18 column (4.6 mm x 250 mm, 5 microm) was used as the 
      analytical column maintained at 25 degrees C. The mobile phase consisted of 10 
      mmol x L(-1) CH3COONH4 and CH3OH (25:75). Flow rate was 1 mL x min(-1). 
      Electrospray ionization (ESI) was carried out. The ESI ion source was set in 
      positive ion polasity mode. The selective ion monitoring (SIM) was set at m/z 
      265.1 and 249.2. RESULT: The linearity ranged from 2 to 5000 ng x mL(-1) (r = 
      0.9991). The detection of oxymatrine and its metabolite were 0.6 and 0.3 ng x 
      mL(-1). The RSD(%) within day and between day was less than 4.7%. The recovery of 
      this method was more than 96.5%. The disposition was conformed to a 
      two-compartment model. The T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of oxymatrine 
      were (5.5+/-1.58) h, (1.0+/-0.30) h, (2418.3 +/-970.78) ng x mL(-1), (3.2+/-0.64) 
      h, (5797.4+/-908.16) ng x mL(-1) x h accordingly. The corresponding T(1/2), Tmax, 
      Cmax, MRT, AUC(0-->24 h) of matrine were (9.8+/-2.77) h, (1.9+/-1.09) h, 
      (1532.4+/-494.86) ng x mL(-1), (4.4+/-1.97) h, (5530.5+/-1042.65) ng x mL(-1) x 
      h. CONCLUSION: This assay was highly sensitive, rapid, simple and specific enough 
      for determining concentrations of oxymatrine and its metabolite matrine in plasma 
      of beagle dog.
FAU - Wang, Su-jun
AU  - Wang SJ
AD  - Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, 
      Nanjing 210038, China.
FAU - Wang, Guang-ji
AU  - Wang GJ
FAU - Li, Xiao-tian
AU  - Li XT
FAU - Ma, Ren-ling
AU  - Ma RL
FAU - Sun, Jian-guo
AU  - Sun JG
FAU - Sheng, Long-sheng
AU  - Sheng LS
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Alkaloids)
RN  - 0 (Quinolizines)
RN  - 85U4C366QS (oxymatrine)
RN  - 0 (Matrines)
SB  - IM
MH  - Administration, Oral
MH  - Alkaloids/blood/isolation & purification/*pharmacokinetics
MH  - Animals
MH  - Area Under Curve
MH  - Chromatography, Liquid
MH  - Dogs
MH  - Male
MH  - *Plants, Medicinal/chemistry
MH  - Quinolizines
MH  - *Sophora/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Matrines
EDAT- 2005/02/18 09:00
MHDA- 2005/07/20 09:00
CRDT- 2005/02/18 09:00
PHST- 2005/02/18 09:00 [pubmed]
PHST- 2005/07/20 09:00 [medline]
PHST- 2005/02/18 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2005 Jan;30(2):133-6.