PMID- 15716599
OWN - NLM
STAT- MEDLINE
DCOM- 20050711
LR  - 20190816
IS  - 1011-8934 (Print)
IS  - 1598-6357 (Electronic)
IS  - 1011-8934 (Linking)
VI  - 20
IP  - 1
DP  - 2005 Feb
TI  - Molecular cytogenetic analysis of gene rearrangements in childhood acute 
      lymphoblastic leukemia.
PG  - 36-41
AB  - The aims of this study were to estimate the incidences of BCR/ABL, MLL, TEL/AML1 
      rearrangements, and p16 deletions in childhood acute lymphoblastic leukemia 
      (ALL), to identify new abnormalities, and to demonstrate the usefulness of 
      interphase fluorescence in situ hybridization (FISH). We performed G-banding 
      analysis and FISH using probes for BCR/ABL, MLL, TEL/AML1 rearrangements, and p16 
      deletions on 65 childhood ALL patients diagnosed and uniformly treated at a 
      single hospital. Gene rearrangements were identified in 73.8% of the patients 
      using the combination of G-banding and FISH, while the chromosomal abnormalities 
      were identified in 49.2% using G-banding alone. Gene rearrangements were 
      disclosed by FISH in 24 (72.7%) of 33 patients with normal karyotype or no 
      mitotic cell in G-banding. Among the gene rearrangements detected by FISH, the 
      most common gene rearrangement was p16 deletion (20.3%) and the incidences of 
      others were 14.1% for TEL/AML1, 11.3% for MLL, and 1.8% for BCR/ABL 
      translocations. Infrequent or new aberrations such as AML1 amplification, MLL 
      deletion, ABL deletion, and TEL/AML1 fusion with AML1 deletion were also 
      observed. We established the rough incidences of gene rearrangements in childhood 
      ALL, found new abnormalities and demonstrated the diagnostic capability of 
      interphase FISH to identify cryptic chromosome aberrations.
FAU - Woo, Hee Yeon
AU  - Woo HY
AD  - Department of Laboratory Medicine, Sungkyunkwan University, School of Medicine, 
      Seoul, Korea.
FAU - Kim, Dae Won
AU  - Kim DW
FAU - Park, Hyosoon
AU  - Park H
FAU - Seong, Ki Woong
AU  - Seong KW
FAU - Koo, Hong Hoe
AU  - Koo HH
FAU - Kim, Sun Hee
AU  - Kim SH
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Korean Med Sci
JT  - Journal of Korean medical science
JID - 8703518
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-AML1 fusion protein)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Core Binding Factor Alpha 2 Subunit
MH  - Cyclin-Dependent Kinase Inhibitor p16/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Gene Deletion
MH  - *Gene Rearrangement
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Interphase
MH  - Male
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Proto-Oncogenes/*genetics
MH  - Transcription Factors/*genetics
MH  - Treatment Outcome
PMC - PMC2808572
EDAT- 2005/02/18 09:00
MHDA- 2005/07/12 09:00
PMCR- 2005/02/01
CRDT- 2005/02/18 09:00
PHST- 2005/02/18 09:00 [pubmed]
PHST- 2005/07/12 09:00 [medline]
PHST- 2005/02/18 09:00 [entrez]
PHST- 2005/02/01 00:00 [pmc-release]
AID - 200502036 [pii]
AID - 10.3346/jkms.2005.20.1.36 [doi]
PST - ppublish
SO  - J Korean Med Sci. 2005 Feb;20(1):36-41. doi: 10.3346/jkms.2005.20.1.36.