PMID- 15717689
OWN - NLM
STAT- MEDLINE
DCOM- 20050323
LR  - 20191026
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 81
IP  - 1
DP  - 2005 Jan
TI  - Incidence of diffuse large B-cell lymphoma of germinal center B-cell origin in 
      whole diffuse large B-cell lymphoma: tissue fluorescence in situ hybridization 
      using t(14;18) compared with immunohistochemistry.
PG  - 48-57
AB  - Diffuse large B-cell lymphoma (DLBCL) can be divided into prognostically 
      important categories such as germinal center B (GCB)-like and non-GCB-like 
      groups. The t(14;18)(q32;q21) translocation defines a unique subset of DLBCL 
      cases with a GCB gene expression profile. Two-color fluorescence in situ 
      hybridization (FISH) analysis was applied to detect t(14;18) (q32;q21) in the 
      nuclei of paraffin-embedded tissue sections from 61 patients with de novo DLBCL. 
      Nine (15%) of 61 cases had a positive pattern. Fifty-seven cases were 
      subclassified in an immunohistochemical study with anti-CD10, anti-bcl-6, and 
      anti-MUM1 antibodies. In this classification, 21 cases (37%) were placed in the 
      GCB group, and 36 (63%) were placed in the non-GCB group. There was a discrepancy 
      between t(14;18) occurrence and bcl-2 protein expression. Bcl-2 protein 
      expression was positive in 40 (67%) of 60 cases. The expression of bcl-2 protein 
      in the GCB and non-GCB groups was not significantly different: 15 (71%) of 21 
      cases in the GCB group and 24 (67%) of 36 cases in the non-GCB group tested 
      positive. We found no difference between the FISH-positive and FISH-negative 
      groups in overall survival time (P = .6019, log-rank test). The overall survival 
      rates of GCB and non-GCB groups did not differ significantly by 
      immunohistochemical classification (P = .5399, log-rank test). Overall survival 
      was significantly longer in the group with a low International Prognostic Index 
      (IPI) score than in the group with a high IPI score (P = .0002, log-rank test). 
      Our results suggest that immunohistochemical study and cytogenetic study with 
      t(14;18) FISH cannot predict the clinical outcomes of DLBCL patients. A study 
      with a larger number of patients may show a difference in clinical outcomes 
      between FISH-positive and FISH-negative groups and between GCB and non-GCB 
      groups.
FAU - Hirose, Yuko
AU  - Hirose Y
AD  - Department of Hematology and Immunology, Kanazawa Medical University, Ishikawa, 
      Japan. y-hirose@kanazawa-med.ac.jp
FAU - Masaki, Yasufumi
AU  - Masaki Y
FAU - Karasawa, Hiromi
AU  - Karasawa H
FAU - Shimoyama, Kumiko
AU  - Shimoyama K
FAU - Fukushima, Toshihiro
AU  - Fukushima T
FAU - Kawabata, Hiroshi
AU  - Kawabata H
FAU - Ogawa, Noriyoshi
AU  - Ogawa N
FAU - Wano, Yuji
AU  - Wano Y
FAU - Ozaki, Mamoru
AU  - Ozaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosomes, Human, Pair 14
MH  - *Chromosomes, Human, Pair 18
MH  - Female
MH  - Germinal Center/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Incidence
MH  - *Lymphoma, B-Cell/genetics/mortality/pathology
MH  - *Lymphoma, Large B-Cell, Diffuse/genetics/mortality/pathology
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 2005/02/19 09:00
MHDA- 2005/03/24 09:00
CRDT- 2005/02/19 09:00
PHST- 2005/02/19 09:00 [pubmed]
PHST- 2005/03/24 09:00 [medline]
PHST- 2005/02/19 09:00 [entrez]
AID - 10.1532/ijh97.04102 [doi]
PST - ppublish
SO  - Int J Hematol. 2005 Jan;81(1):48-57. doi: 10.1532/ijh97.04102.