PMID- 15717751
OWN - NLM
STAT- MEDLINE
DCOM- 20050304
LR  - 20180217
IS  - 0001-5547 (Print)
IS  - 0001-5547 (Linking)
VI  - 49
IP  - 1
DP  - 2005 Jan-Feb
TI  - HER-2/neu evaluation by fluorescence in situ hybridization on destained cytologic 
      smears from primary and metastatic breast cancer.
PG  - 27-30
AB  - OBJECTIVE: To evaluate HER-2/neu amplification by fluorescence in situ 
      hybridization (FISH) (HER-2/neu by FISH) on archival cytologic smears stained 
      with May-Grunwald-Giemsa (MGG) stain. STUDY DESIGN: Cytologic specimens from 69 
      breast cancer lesions (48 primary and 21 metastatic), stained with MGG stain for 
      routine diagnostic cytology, were destained and subjected to HER-2/neu by FISH. 
      Fifteen of the 69 samples were also evaluated by FISH on paired fresh smears. 
      RESULTS: HER-2/neu by FISH was successfully assayed in 25 of the 48 primary 
      tumors and in 15 of the 21 metastatic lesions, corresponding to an overall 
      feasibility of 58%. These cases had been archived between 1 month and 10 years 
      prior to FISH analysis. Eight of the 25 primary and 5 of the 15 metastatic tumors 
      were amplified. In 15 of the 40 evaluable cases, HER-2/neu was also assessed on 
      the corresponding fresh smears: 8 tumors were amplified and 7 unamplified on both 
      destained MGG and fresh smears. CONCLUSION: HER-2/neu can be detected by FISH on 
      routinely MGG-stained cytologic slides. This approach allows HER-2/neu evaluation 
      whenever histologic sections or fresh cytologic material are not available. In 
      these cases, HER-2/neu assessment on destained cytologic smears plays a role in 
      the selection of targeted therapy.
FAU - Nizzoli, Rita
AU  - Nizzoli R
AD  - Department of Medical Oncology, University Hospital, Parma, Italy. 
      rnizzoli@ao.pr.it
FAU - Guazzi, Annamaria
AU  - Guazzi A
FAU - Naldi, Nadia
AU  - Naldi N
FAU - Fraciosi, Vittorio
AU  - Fraciosi V
FAU - Bozzetti, Cecilia
AU  - Bozzetti C
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
SB  - IM
MH  - Biopsy, Needle
MH  - Breast Neoplasms/*genetics/*pathology
MH  - Carcinoma/*genetics/*secondary
MH  - Female
MH  - *Genes, erbB-2
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Retrospective Studies
MH  - Staining and Labeling
EDAT- 2005/02/19 09:00
MHDA- 2005/03/05 09:00
CRDT- 2005/02/19 09:00
PHST- 2005/02/19 09:00 [pubmed]
PHST- 2005/03/05 09:00 [medline]
PHST- 2005/02/19 09:00 [entrez]
AID - 10.1159/000326091 [doi]
PST - ppublish
SO  - Acta Cytol. 2005 Jan-Feb;49(1):27-30. doi: 10.1159/000326091.