PMID- 15718291
OWN - NLM
STAT- MEDLINE
DCOM- 20050920
LR  - 20191210
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 19
IP  - 7
DP  - 2005 Jul
TI  - Follicle-stimulating hormone induction of ovarian insulin-like growth 
      factor-binding protein-3 transcription requires a TATA box-binding protein and 
      the protein kinase A and phosphatidylinositol-3 kinase pathways.
PG  - 1837-48
AB  - The current study was done to elucidate the mechanism of the FSH stimulation of 
      IGF-binding protein 3 (IGFBP-3) expression and map the FSH response element on 
      the pig IGFBP-3 promoter. Forskolin induced IGFBP-3 reporter activity in 
      transiently transfected granulosa cells. The protein kinase A (PKA) inhibitor 
      [N-[2-(p-bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl] (and 
      cotransfection with a PKA inhibitor expression vector), the 
      phosphatidylinositol-3 kinase inhibitor 
      [2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one], and the ERK inhibitor 
      [1,4-diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)butadiene], all blocked FSH 
      stimulation. Use of serial deletion constructs and site-directed mutagenesis show 
      that a TATA box-binding protein site is required for FSH stimulation and that a 
      specific protein 1 (Sp1) site is required for basal transcription. Gel shift 
      assays of nuclear protein with a -61/-25 probe detected four protein-DNA 
      complexes, with bands I and II having significantly higher intensities in 
      FSH-treated cells than in controls. Mutation of the Sp1 site prevented formation 
      of bands I and II whereas mutation of the TATA box-binding protein site prevented 
      formation of band IV. Use of specific antibodies showed that Sp1 participates in 
      formation of band I, Sp3 band II, and p300 in both I and II. Band III was 
      nonspecifically competed out. We conclude that FSH stimulation of IGFBP-3 
      transcription is mediated by cAMP via the PKA pathway and requires the P1-3 
      kinase and likely the MAPK pathways.
FAU - Ongeri, Elimelda Moige
AU  - Ongeri EM
AD  - Pennsylvania State University, College of Medicine, Hershey Medical Center, 500 
      University Drive, Hershey, PA 17033, USA.
FAU - Verderame, Michael F
AU  - Verderame MF
FAU - Hammond, James M
AU  - Hammond JM
LA  - eng
GR  - HD24564/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050217
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Insulin-Like Growth Factor Binding Protein 3)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (TATA-Box Binding Protein)
RN  - 0 (Transcription Factors)
RN  - 1F7A44V6OU (Colforsin)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors/metabolism
MH  - Female
MH  - Follicle Stimulating Hormone/pharmacology/*physiology
MH  - Granulosa Cells/drug effects/enzymology/*metabolism
MH  - Insulin-Like Growth Factor Binding Protein 3/*genetics
MH  - Mutagenesis, Site-Directed
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Promoter Regions, Genetic/genetics
MH  - Response Elements
MH  - Sequence Deletion
MH  - *Signal Transduction
MH  - Swine
MH  - TATA Box/genetics
MH  - TATA-Box Binding Protein/*metabolism
MH  - Transcription Factors/metabolism
MH  - *Transcriptional Activation
EDAT- 2005/02/19 09:00
MHDA- 2005/09/21 09:00
CRDT- 2005/02/19 09:00
PHST- 2005/02/19 09:00 [pubmed]
PHST- 2005/09/21 09:00 [medline]
PHST- 2005/02/19 09:00 [entrez]
AID - me.2004-0487 [pii]
AID - 10.1210/me.2004-0487 [doi]
PST - ppublish
SO  - Mol Endocrinol. 2005 Jul;19(7):1837-48. doi: 10.1210/me.2004-0487. Epub 2005 Feb 
      17.