PMID- 15718418
OWN - NLM
STAT- MEDLINE
DCOM- 20050712
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 105
IP  - 11
DP  - 2005 Jun 1
TI  - Identification and characterization of undifferentiated mast cells in mouse bone 
      marrow.
PG  - 4282-9
AB  - Sequential immunomagnetic isolation with 2 monoclonal antibodies was used to 
      purify and characterize an undifferentiated mast cell in adult mouse bone marrow 
      that had not been previously recognized. This cell represents 0.02% of the cells 
      in the bone marrow, is CD34(+), CD13(+), and c-kit(+), and does not express 
      FcepsilonRI. However, by polymerase chain reaction (PCR) the cell contains 
      message for the alpha and beta subunits of FcepsilonRI, mast cell-specific 
      proteases, and carboxypeptidase A. Morphologically, this cell has a large 
      nucleus, little cytoplasm, few cytoplasmic organelles, and no cytoplasmic 
      granules. In vitro, in the presence of interleukin-3 (IL-3) and stem cell factor 
      (SCF) these cells differentiate only into a granulated mast cell that now 
      expresses CD13, c-kit, mast cell-specific gangliosides, FcepsilonRI, and binds 
      immunoglobulin E (IgE). When injected into lethally irradiated mice, these cells 
      are able to reconstitute the mast cell population in the spleen.
FAU - Jamur, Maria Celia
AU  - Jamur MC
AD  - Faculdade de Medicina de Ribeirao Preto, Departamento de Biologia Celular e 
      Molecular e Bioagentes Patogenicos, Universidade de Sao Paulo, Ribeirao Preto, 
      SP, Brasil.
FAU - Grodzki, Ana Cristina G
AU  - Grodzki AC
FAU - Berenstein, Elsa H
AU  - Berenstein EH
FAU - Hamawy, Majed M
AU  - Hamawy MM
FAU - Siraganian, Reuben P
AU  - Siraganian RP
FAU - Oliver, Constance
AU  - Oliver C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050217
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD34)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, IgE)
RN  - EC 3.4.11.2 (CD13 Antigens)
SB  - IM
MH  - Animals
MH  - Antigens, CD34
MH  - Bone Marrow Cells
MH  - CD13 Antigens
MH  - Cell Differentiation
MH  - Female
MH  - Immunomagnetic Separation
MH  - Immunophenotyping
MH  - Male
MH  - Mast Cells/chemistry/*cytology/transplantation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Organelles/ultrastructure
MH  - RNA, Messenger/analysis
MH  - Receptors, IgE/genetics
EDAT- 2005/02/19 09:00
MHDA- 2005/07/13 09:00
CRDT- 2005/02/19 09:00
PHST- 2005/02/19 09:00 [pubmed]
PHST- 2005/07/13 09:00 [medline]
PHST- 2005/02/19 09:00 [entrez]
AID - S0006-4971(20)53466-6 [pii]
AID - 10.1182/blood-2004-02-0756 [doi]
PST - ppublish
SO  - Blood. 2005 Jun 1;105(11):4282-9. doi: 10.1182/blood-2004-02-0756. Epub 2005 Feb 
      17.