PMID- 15719361
OWN - NLM
STAT- MEDLINE
DCOM- 20050816
LR  - 20191210
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 26
IP  - 9
DP  - 2005 May
TI  - Capillary zone electrophoresis of amino acids on a hybrid 
      poly(dimethylsiloxane)-glass chip.
PG  - 1849-60
AB  - Poly(dimethylsiloxane) (PDMS)-PDMS and hybrid PDMS-glass devices have been 
      characterized and compared in terms of current-voltage linearity, contact angle, 
      electroosmotic velocity, electroosmotic mobility, and electrokinetic potential in 
      dependence on the surface treatment. The hybrid PDMS-glass microfluidic devices 
      have further been tested as on-chip capillary electrophoresis systems for the 
      separation of fluorescently labeled amino acids. It has been demonstrated that 
      different methods of surface pretreatment of the PDMS-glass devices result in 
      significantly different separation performance, with plate numbers varying from 
      650 to 57 000 in dependence on the surface state and the nature of the amino 
      acids. Electrophoretic separations of amino acids have been achieved within tens 
      of seconds with detection limits of less than 2 microM (approximately 2 x 10(-16) 
      to 2.5 x 10(-16) mol quantities at injection volumes of 110-120 pL). The detected 
      amounts of fluorescein isothiocyante (FITC)-amino acids are at least ten times 
      lower, since the amino acid:FITC ratio is 10:1 mol. The results demonstrate the 
      perspective of such hybrid PDMS-glass microfluidic systems and the methods to 
      modify their surfaces for on-chip separation methods for biomolecules.
FAU - Mourzina, Yulia
AU  - Mourzina Y
AD  - Institute of Thin Films and Interfaces, Research Center Julich, D-52425 Julich, 
      Germany. y.mourzina@fz-juelich.de
FAU - Steffen, Alfred
AU  - Steffen A
FAU - Kalyagin, Dmitry
AU  - Kalyagin D
FAU - Carius, Reinhard
AU  - Carius R
FAU - Offenhausser, Andreas
AU  - Offenhausser A
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Amino Acids)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Amino Acids/*analysis
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Electrophoresis, Capillary/*instrumentation
MH  - Electrophoresis, Microchip/*instrumentation
MH  - Glass/*chemistry
MH  - Silicones/*chemistry
EDAT- 2005/02/19 09:00
MHDA- 2005/08/17 09:00
CRDT- 2005/02/19 09:00
PHST- 2005/02/19 09:00 [pubmed]
PHST- 2005/08/17 09:00 [medline]
PHST- 2005/02/19 09:00 [entrez]
AID - 10.1002/elps.200410295 [doi]
PST - ppublish
SO  - Electrophoresis. 2005 May;26(9):1849-60. doi: 10.1002/elps.200410295.