PMID- 15719407
OWN - NLM
STAT- MEDLINE
DCOM- 20050705
LR  - 20111117
IS  - 1527-6465 (Print)
IS  - 1527-6465 (Linking)
VI  - 11
IP  - 3
DP  - 2005 Mar
TI  - Microchimerism after liver transplantation: absence of rejection without 
      abrogation of anti-donor cytotoxic T-lymphocyte-mediated alloreactivity.
PG  - 290-7
AB  - Microchimerism (MC) is defined by the persistence of <1% circulating donor cells 
      resulting from cell migration from the graft; MC may play a role in the induction 
      of unresponsiveness to allogeneic tissues, or may be merely the consequence of 
      the graft's acceptance following immunosuppression. To analyze early MC (7 
      patients) and late MC (12 patients) following a liver transplantation, we 
      designed a sensitive and semiquantitative nested polymerase chain reaction (PCR) 
      protocol based on the detection of incompatible human leukocyte antigen 
      (HLA)-DRB1 donor alleles. MC was measured in multiple PCR samples and expressed 
      as percent positive PCRs / time point. The detection level was 1 donor cell / 
      10(5) patient cells. All patients had detectable early MC, ranging from 5 to 100% 
      positive PCRs in the 1st 3 months after transplantation. The kinetic analysis 
      demonstrated that MC decreased during the 1st year in 6 of 7 patients. All of the 
      4 patients with the lowest MC had rejection episodes, vs. none among the 3 
      patients with MC >50%. However, cytotoxic T-lymphocyte reactivity (CTL) against 
      HLA class I donor antigens could be demonstrated 1 year posttransplant in 2 
      patients with a high level of early MC. MC is a dynamic process, which is easily 
      detectable <3 months after liver transplantation. In conclusion, a correlation 
      between the level of early MC and the absence of rejection episodes was observed. 
      However, high levels of early MC did not abrogate the persistence of an 
      alloreactive response measured in vitro 1 year after transplantation, which 
      suggests that MC did not lead to clonal deletion of donor-specific CTL.
FAU - Bettens, Florence
AU  - Bettens F
AD  - Immunology and Transplant Unit, Geneva University Hospital, 1211 Geneva, 
      Switzerland.
FAU - Tiercy, Jean-Marie
AU  - Tiercy JM
FAU - Campanile, Nathalie
AU  - Campanile N
FAU - Giostra, Emiliano
AU  - Giostra E
FAU - Majno, Pietro
AU  - Majno P
FAU - Rubbia, Laura
AU  - Rubbia L
FAU - Roosnek, Eddy
AU  - Roosnek E
FAU - Mentha, Gilles
AU  - Mentha G
FAU - Villard, Jean
AU  - Villard J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Liver Transpl
JT  - Liver transplantation : official publication of the American Association for the 
      Study of Liver Diseases and the International Liver Transplantation Society
JID - 100909185
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Follow-Up Studies
MH  - Graft Rejection/*immunology
MH  - HLA-DR Antigens/genetics
MH  - HLA-DRB1 Chains
MH  - Histocompatibility Testing
MH  - Humans
MH  - Isoantibodies/*blood
MH  - Liver Transplantation/*immunology
MH  - Polymerase Chain Reaction/methods
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Time Factors
MH  - *Transplantation Chimera
EDAT- 2005/02/19 09:00
MHDA- 2005/07/06 09:00
CRDT- 2005/02/19 09:00
PHST- 2005/02/19 09:00 [pubmed]
PHST- 2005/07/06 09:00 [medline]
PHST- 2005/02/19 09:00 [entrez]
AID - 10.1002/lt.20360 [doi]
PST - ppublish
SO  - Liver Transpl. 2005 Mar;11(3):290-7. doi: 10.1002/lt.20360.