PMID- 15719610
OWN - NLM
STAT- MEDLINE
DCOM- 20050318
LR  - 20161021
IS  - 1110-4902 (Print)
VI  - 10
IP  - 2
DP  - 2003
TI  - Immunological studies on mice vaccinated with irradiated cercaria and IL-12 
      against Schistosoma mansoni infection.
PG  - 39-48
AB  - Protective immunity against Schistosoma mansoni infection correlates with 
      increased levels of IgE and blood eosinophilia which are considered as markers of 
      anti-parasitic cell-mediated immunity. IL-5 participates as well in the induction 
      and regulation of IgE and eosinophilia, consequently in the development of 
      acquired immunity. Swiss Webster female mice were subcutaneously injected with 
      either 50 microg of gamma-irradiated cercarial homogenate (400 Gy) twice weekly 
      for three weeks alone or plus a single dose of IL-12 (0.8 ng/Kg). The efficiency 
      of immunization regimens were assessed 45 days post infection with 100 live 
      cercariae/mouse by the number of worm burden, ova count, production of IL-5, 
      eosinophils, and IgE levels in the vaccinated groups compared with the 
      non-immunized group. The results demonstrated a significant reduction of ova 
      count in the livers of vaccinated groups (57.19 and 40.13%) and worm couples 
      compared with the non -immunized group. Furthermore, a decrease of IL-5 level as 
      well as eosinopenia was recorded in both vaccinated groups. Scanning electron 
      microscope (SEM) of adult worms recovered from the immunized groups revealed 
      marked damage on the tegumental surface in males rather than females as well as 
      constrictions and intensive corrugation of intertubercles.
FAU - el-Gawish, Mona A M
AU  - el-Gawish MA
AD  - National Center for Radiation Research and Technology, Atomic Energy Authority, 
      Cairo, Egypt.
FAU - Moawad, Mervat Abdel Fattah
AU  - Moawad MA
LA  - eng
PT  - Journal Article
PL  - Egypt
TA  - Egypt J Immunol
JT  - The Egyptian journal of immunology
JID - 9816016
RN  - 0 (Antigens, Helminth)
RN  - 0 (Interleukin-5)
RN  - 187348-17-0 (Interleukin-12)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/administration & dosage
MH  - Eosinophils/immunology
MH  - Female
MH  - Gamma Rays
MH  - Immunoglobulin E/blood
MH  - Interleukin-12/*administration & dosage
MH  - Interleukin-5/blood
MH  - Leukocyte Count
MH  - Male
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Schistosoma mansoni/*immunology/radiation effects/ultrastructure
MH  - Schistosomiasis mansoni/*immunology/parasitology/prevention & control
MH  - Vaccination
EDAT- 2005/02/22 09:00
MHDA- 2005/03/19 09:00
CRDT- 2005/02/22 09:00
PHST- 2005/02/22 09:00 [pubmed]
PHST- 2005/03/19 09:00 [medline]
PHST- 2005/02/22 09:00 [entrez]
PST - ppublish
SO  - Egypt J Immunol. 2003;10(2):39-48.